Evaluation of bioartificial renal tubule device pr

Authors: Hiroo Takahashi Kaichiro Sawada Takatoshi Kakuta Takao Suga Kazuya Hanai Genta Kanai Satoshi Fujimura Noriyuki Sanechika Toshiro Terachi Masafumi Fukagawa Akira Saito

Publish Date: 2013/05/12

Volume: 16, Issue: 3, Pages: 368-375

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Abstract

Bioartificial renal tubule devices BTD use cell therapy to improve conditions commonly observed in recipients of artificial kidneys for treatment of kidney diseases We previously reported significant improvement of the condition of acute kidney injury AKI animals after treatment with BTD prepared with lifespanextended human renal proximal tubular cells hRPTEC However a major obstacle to use of BTD for patients is their biological safety because hRPTEC are cultured in medium containing fetal calf serum To establish the biological safety of BTD we prepared BTD with lifespanextended hRPTEC cultured in a newly developed serumfree medium and compared these with BTD prepared with hRPTEC cultured in serumcontaining conventional medium Lifespanextended hRPTEC cultured in serumfree medium hRPTECSFM can proliferate similar to hRPTEC cultured in serumcontaining conventional medium hRPTECCM Comparison of leakage and of reabsorption of small molecules for BTD prepared with hRPTECSFM BTDSFM with those for our previous BTD prepared with hRPTECCM BTDCM showed transportation in these two types of BTD was almost identical When AKI goats were treated with BTDSFM for 26 h increase of survival time and reduction of cytokine expression in blood cells were almost same as for AKI goats treated with BTDCM Quantification of the expression of some genes of hRPTEC in BTD revealed significant changes during BTD treatment for AKI goats In conclusion lifespanextended hRPTECSFM work as well as hRPTECCM and the biological safety of BTD for patients could be elevated without loss of function by preparation from hRPTECSFMThis work was supported by the Regional Innovation Creation RD Program ref 20R3003 of the Ministry of Economy Trade and Industry of Japan We thank the Kihara Memorial Yokohama Foundation for the Advancement of Life Science for their support and the Animal Care Center and Education and Research Support Center Tokai University School of Medicine for their technical assistance

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