First isolation and establishment of in vitro cult

Authors: M Namavari S Ezhdehakoshpour G R Habibi A Rahimian F Namazi

Publish Date: 2013/05/22

Volume: 39, Issue: 2, Pages: 328-331

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Abstract

Theileria infected cell line was isolated from the prescapular lymph node of an adult crossbred cow Molecular study confirmed this cell line of bovine lymphocyte has been transformed by the Theileria lestoquardi This strain of T lestoquardi designated Ka6 and sheep were inoculated with this strain didn’t show any clinical signs of theileriosis which shows the significance of this cell line to develop a tissueculture vaccine against malignant ovine theileriosis Contrary to accepted belief that the T lestoquardi not capable of causing disease in cattle the present study describes the first isolation and establishment of in vitro culture of T lestoquardiinfected cell line from a naturally infected cow with typical singes of acute theileriosis

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