Molecular and parasitological study of cutaneous l

Authors: Mehdi Mohebali Hossein Darabi Homa Hajjaran Mohammad Reza Shirzadi Moradali Fouladvand Soroor Charehdar Mohammad Taghi Satvat Foroogh Pakbaz Katayoun Vahdat

Publish Date: 2013/12/12

Volume: 39, Issue: 3, Pages: 371-376

PDF Link

Abstract

Cutaneous leishmaniasis CL is one of the most important parasitic disease in Iran CL is distributed among more than half of 31 provinces of Iran Studies on epidemiological aspects of the disease and Leishmania species identification among infected humans are necessary for providing a comprehensive prevention and control program thus this descriptive crosssectional study was conducted on all CL suspected patients who referred to Health Centers of Bushehr province from 2009 to 2012 Physical examinations were carried out in suspected individuals and CL cases were confirmed by microscopical examinations Prepared slides from suspicious cases of CL were fixed with absolute methanol and stained by Giemsa 10  All the Giemsastained slides examined under a light microscope with high magnification 1000× and classified them based on grading of Leishmania parasites DNA from each slide was extracted separately The ribosomal internal transcribed spacer 1 was amplified with specific primers and PCR products were digested by restrict enzymes HaeIII run them in 3  gel agarose for electrophoresis and visualized on a UV transilluminator after staining with ethidium bromide SPSS version 21 was used for data analyses A total of 726 suspected CL cases were referred to Health Centers of Bushehr province from 2009 to 2012 and samples were only prepared from 188 of the patients whereas 43 59  of them were microscopy positive The most frequent of CL was observed in November 14  and December 12  The most distribution of CL lesions were observed on hands 32  feet 26  and face 21  respectively The highest frequency of CL was observed in 1–9 years old 30  Altogether 50  of the patients showed one skin lesion and 2–10 skin lesions were occurred in the remained CL patients Totally 27 out of 43 63  of the Giemsa stained slides were positive by PCR–RFLP assay because all the PCR–RFLP negative slides were prepared 3–4 years ago and kept without cover slip and also observed scarce amastigotes during microscopy observations Leishmania species were identified in 21 desirable slides which 14 of them were L major and 7 of the remained isolates were identified L tropica using PCR–RFLPThis study was financially supported by ViceChancellors for Education and Research of School of Public Health Med Sciences/University of Tehran as well as Bushehr University of Medical Sciences We wish to thank Mr Z Zarei from MeshkinShahr Research station Tehran of Medical Sciences The authors are thankful to our other colleagues for laboratory and field activities

Keywords: