Molecular Features of Follicular Variant Papillary

Authors: Guven Guney Gaye Guler Tezel Kemal Kosemehmetoglu Engin Yilmaz Serdar Balci Reyhan Ersoy Bekir Cakir Gulnur Guler

Publish Date: 2013/11/27

Volume: 25, Issue: 3, Pages: 241-247

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Abstract

We aimed to compare the genetic background of different areas in follicular variant papillary thyroid carcinomas FVPTC with or without classical nuclear changes Sixteen cases of FVPTC were included in our study All tumors were well demarcated from surrounding thyroid tissue and had both areas with nuclear features WNF and areas without nuclear features WONF of papillary carcinoma DNA is obtained by laser microdissection from WNF and WONF areas of each case Point mutations for NRAS codon 61 HRAS codon 61 and BRAF were investigated by direct sequencing In 11 cases reverse transcription PCR was performed for the presence of PAX8PPARɣ and RET/PTC1–3 gene rearrangements Point mutation for NRAS codon 61 was also studied in 15 colloidal nodules Seven cases 44  showed at least one mutation two cases 13  revealed the same mutation in both WNF and WONF areas while in the rest only WNF areas were mutated None of the studied 11 cases demonstrated RET/PTC1–3 gene rearrangement and in only one case PAX8PPARɣ gene rearrangement was found Six cases 38  showed NRAS codon 61 mutation involving only WNF areas in five cases and both WNF and WONF areas in one case Neither HRAS codon 61 nor BRAF mutations were present Fifteen colloidal nodules were also wild type for NRAS codon 61 Our findings suggest that NRAS codon 61 point mutations and PAX8PPARɣ gene rearrangement play a role in the FVPTC pathogenesis and may be established before the morphological/phenotypical features fully develop

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