Journal Title
Title of Journal: J Mol Hist
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Abbravation: Journal of Molecular Histology
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Publisher
Springer Netherlands
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Authors: Meng Lu Jiao Wu Feng He XiLong Wang Can Li ZhiNan Chen Huijie Bian
Publish Date: 2014/12/02
Volume: 46, Issue: 1, Pages: 79-91
Abstract
Overexpression of CD147/basigin in hepatic cells promotes the progression of hepatocellular carcinoma HCC Whether CD147 also expressed in liver nonparenchymal cells and associated with HCC development was unknown The aim of the study was to explore timedependent cell expression patterns of CD147 in a widely accepted Ndiethylnitrosamine/phenobarbital DEN/PBinduced HCC mouse model Liver samples collected at month 1–12 of postDEN/PB administration were assessed the localization of CD147 in hepatocytes endothelial cells hepatic stellate cells and macrophages Immunohistochemistry analysis showed that CD147 was upregulated in liver tumors during month 1–8 of DEN/PB induction Expression of CD147 was positively correlated with cytokeratin 18 a hepatocyte marker r = 07857 P = 00279 CD31 r = 09048 P = 00046 an endothelial cell marker and CD68 a macrophage marker r = 07619 P = 00368 A significant correlation was also observed between CD147 and alphasmooth muscle actin r = 08857 P = 00333 at DEN/PB initiation and early stage of tumor formation Immunofluorescence and fluorescence in situ hybridization showed that CD147 coexpressed with cytokeratin 18 CD31 alphasmooth muscle actin and CD68 Moreover there existed positive correlations between CD147 and microvessel density r = 07857 P = 00279 CD147 and Ki67 r = 09341 P = 00022 in the development of DEN/PBinduced HCC In conclusion our results demonstrated that CD147 was upregulated in the liver parenchymal and mesenchymal cells and involved in angiogenesis and tumor cell proliferation in the development of DEN/PBinduced HCC
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