Authors: S Sri Shalini J Kurian J W C Wong
Publish Date: 2014/01/09
Volume: 12, Issue: 3, Pages: 1107-1122
Abstract
Leachate from the municipal solid waste MSW landfills contains high concentration of ammoniacal nitrogen that is a major toxic pollutant that has a great threat to environment Among the processes available for the removal of ammoniacal nitrogen one process is a combination of partial nitrification and anaerobic ammonium oxidation anammox process It requires aerobic ammoniumoxidising bacteria AOB and anaerobic ammoniumoxidising bacteria AnAOB This paper presents the feasibility of enriching the AOB and AnAOB in 100mL and 25L batch reactors from fresh and mined MSW and leachate under aerobic and anaerobic conditions with varying feedtoseed ratio The AOB and AnAOB activity was monitored by measuring the intermediates such as hydroxylamine and hydrazine along with variations in ammoniacal nitrogen nitrite nitrogen and nitrate nitrogen concentrations in the reactor contents The formation of intermediates such as hydroxylamine and hydrazine and ammoniacal nitrogen transformation data confirmed the enrichment of AOB and AnAOB Further AOB and AnAOB were validated by most probable number test and scanning electron microscopy analysis respectively DNA extraction polymerase chain reaction amplification and sequencing analysis authenticated the AnAOB as Candidatus Brocadia anammoxidansThe support of the All India Council for Technical Education to carry out this study in the form of National Doctoral Fellowship to the corresponding author is gratefully acknowledged The author wishes to thank Dr Ammaiyappan Selvam and Dr Su Yun Xu Hong Kong Baptist University for their help in the molecular analysis of the bacteria
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