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Title of Journal: Int J Environ Sci Technol

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Abbravation: International Journal of Environmental Science and Technology

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Springer Berlin Heidelberg

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DOI

10.1002/9781118188040.fmatter

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1735-2630

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Bacterial consortium expressing surface displayed

Authors: S Thiengmag S Chuencharoen N Thasana W Whangsuk W Jangiam S Mongkolsuk S Loprasert
Publish Date: 2016/06/07
Volume: 13, Issue: 8, Pages: 2067-2078
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Abstract

The lipA gene encoding a solventtolerant extracellular lipase from Proteus sp SW1 was displayed on the cell surface of Escherichia coli by fusing it to an antigen 43 anchoring motif The display of LipA on the Escherichia coli cell surface was directly confirmed by immunofluorescence microscopy and flow cytometry After 6 days of incubation in media containing 1  used cooking oil an Escherichia coli strain expressing surface displayed lipase was able to degrade 27  of the oil The biosurfactant pseudopyronine B was purified from culture supernatants of Pseudomonas sp SL31 Its critical micelle concentration was determined to be 1400 mg/l and the surfactant was stable within a temperature range from 0 to 120 °C and a pH range of 3–11 Pseudopyronine Bcontaining crude media extracts efficiently removed up to 51  of the cadmium from contaminated water We demonstrated the oil degradation ability of the mixed culture of four bacterial strains namely the recombinant Escherichia coli expressing cell surface displayed lipase pKKJlipA Histagged lipase pETlipA extracellular lipaseproducing Proteus sp SW1 and pseudopyronine Bproducing Pseudomonas sp SL31 by culturing in LB media containing 1  oil The consortium degraded 29  of oil in one day and reached 84  after 7 daysThis research work was supported in part by a grant from the Chulabhorn Research Institute and Center of Excellence on Environmental Health and Toxicology Thailand Special thanks go to Piyajit Watcharasit for Texas redconjugated antirabbit IgG antibody Nuchanart Rangkadilok and Supachai Ritruechai for preparative HPLC Amnart Khongmanee for his kind assistance with fluorescence microscopes James Dubbs for critical reading and discussion Pakwilai Chouichit for technical assistance and also to Piyapol Munpiyamit for photograph preparation We appreciate the funding by the Royal Golden Jubilee PhD Program PHD/0144/2554 to Sirinthra Thiengmag

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