Dominance of Hydrogenotrophic Methanogens at the P

Authors: Gian Powell B Marquez Wolfgang T Reichardt Rhodora V Azanza Deo Florence L Onda Arturo O Lluisma Marco Nemesio E Montaño

Publish Date: 2015/01/23

Volume: 6, Issue: 2, Pages: 201-207

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Abstract

Marine biomasses used in biogasification system are usually treated as terrestrial feedstock after salt removal However biogas production of sea wrack biomass run under thalassic marine conditions and seeded with different inocula cow manure CM marine sediment MS and sea wrackassociated SWA microflora showed the potential for cheaper alternative to freshwater utilization terrestrial conditions To understand how methane yield is affected by the different inoculants under thalassic conditions methanogenic communities in these digesters CM MS SWA were quantified using doublelabeled oligonucleotide probes to a modified fluorescence in situ hybridization at the peak of biogas production Total targeted methanogens were highest in SWA 151 ± 053 × 107 cells ml−1 while lowest in CM 179 ± 020 × 106 cells ml−1 Among all types of targeted methanogens hydrogenotrophs Methanobacteriales and Methanomicrobiales dominated in all digesters CM 732 ± 7  MS 584 ± 80  SWA 612 ± 54  Moreover MS showed mixotrophic Methanosarcinaceae 302 ± 81  codominance suggesting that their population could have influenced the higher methane yield in MSThis work was supported by the Marine Science Institute the DOSTPCAMRD Scholarship the DABiotech through Fucoidan Project and SPEX Project and the DOSTPCAMRD through the PhilHABS project The corresponding author would also like to acknowledge the unwavering support of Engr Joselito P Marquez Alper James Alcaraz Dang Marviluz Espita Joyce Nieva Deanna Luciano Wilfred John Santiañez and the rest of the Seaweed Chemistry and HAB Laboratory staff and the critical discussion technical support and probes provision of Dr Michael Klocke and Dr Ingo Bergmann of the Leibnitz Institut für Agrartechnik Potsdam Germany This is the Marine Science Institute University of the Philippines contribution no 434

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