Simultaneous Saccharification and Fermentation of

Authors: Madhuri Narra Jisha P James Jyoti Divecha Velmurugan Balasubramanian Disha Shah Anil Kurchania Manisha R Harijan

Publish Date: 2016/08/20

Volume: 8, Issue: 4, Pages: 1089-1103

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Abstract

Low strength sodium hydroxide pretreated rice straw was hydrolysed at two different solid loadings ie 10 and 15  w/v with inhouse cellulases IC produced by Aspergillus terreus along with commercial cellulases CC by using batch and fed batch hybrid simultaneous saccharification and fermentation process Optimization of process parameters enhanced the crude cellulase activities ie filter paper βglucosidase and endoglucanase by 16 27 and 22 fold in 6 days as compared to activities obtained on a single substrate ie rice straw Out of four fed batch approaches approach III AIII at 10  solid loading yielded higher ethanol concentrations of 3055 and 2866 g L−1 by using thermo tolerant inhouse yeast strain Kluyveromyces marxianus at 42 °C Combination of 9 FPU g−1 substrate and CC plus 30 CBU of commercial βglucosidase Cβ yielded slightly higher theoretical ethanol yields of 9224  as compared to 8654  obtained from IC+Cβ Dunnett’s Post Hoc Annova test also proved that CC+Cβ were found to be significant in batch as well fed batch experiments as compared to other combinations tried Thus fed batch AIII seems to be an optimistic one to overcome the problems associated with batch mode to achieve higher ethanol yieldsThe authors wish to thank the Director Sardar Patel Renewable Energy Research Institute SPRERI VallabhVidyanagar Gujarat for providing facilities to perform research work at SPRERI Funding from Indian Council of Agricultural Research VVN/EAAI/DRETBCT/2014/1 CRP/VVN/2015/2 and Department of Biotechnology DBT DO No BT/PR10103/PBD/26/131/2007 Government of India New Delhi 110 003 is highly acknowledged

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