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Title of Journal: Opt Rev

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Abbravation: Optical Review

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Springer Japan

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DOI

10.1007/bf02305157

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1349-9432

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Nanoscale energyroute selector consisting of mult

Authors: Ryo Fujii Takahiro Nishimura Yusuke Ogura Jun Tanida
Publish Date: 2015/02/25
Volume: 22, Issue: 2, Pages: 316-321
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Abstract

We report on a nanoscale energyroute selector consisting of multiple fluorescence resonance energy transfer FRET structures switched by external signaling with multiple wavelengths of light In each FRET structure a specific activator molecule is incorporated to a FRET pair of a donor and an acceptor to control the activation of the acceptor Owing to this configuration the FRET structures are switched independently and an energy route is selected Two photoswitchable FRET structures one consists of Alexa Fluor 568 donor Cy5 acceptor and Alexa Fluor 405 activator and the other consists of Alexa Fluor 568 donor Cy55 acceptor and Cy3 activator were constructed using DNA strands modified with fluorescence molecules Switching rates for the individual FRET structures were measured as 64 and 49  respectively An energyroute selector was then assembled with the FRET structures which share a single donor Experimental results demonstrate that the energy route can be changed repeatedly by activation control using three wavelengths of lightThis work was partially supported by a Grantinaid for Scientific Research on Innovative Areas “Nanomedicine Molecular Science” No 2306 from Ministry of Education Culture Sports Science and Technology of Japan and by a Grantinaid for JSPS Fellows Project No 256027 from the Japan Society for the Promotion of Science JSPS

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