Authors: Jing Yuan Xinyi Tang Kai Yin Jie Tian Ke Rui Jie Ma Chaoming Mao Jianguo Chen Liwei Lu Huaxi Xu Shengjun Wang
Publish Date: 2015/03/14
Volume: 62, Issue: 1, Pages: 81-88
Abstract
VP1 protein is the immunodominant capsid protein of enterovirus 71 EV71 which is responsible for large outbreaks of hand foot and mouth disease It has been reported that glucocorticoidinduced tumor necrosis factor receptorrelated protein GITR and its ligand GITRL are involved in modulating both innate and adaptive immune responses In this study a DNA vaccine vector encoding EV71 VP1 gene and mGITRL gene pIRES/VP1/mGITRL was constructed And female Balb/c mice were immunized intramuscularly with the DNA vaccine Compared with the groups immunized with pIRES pIRES/VP1 pIRES/mGITRL and PBS the inoculation of pIRES/VP1/mGITRL induced a higher levels of EV71 VP1specific antibody and specific antibodyforming cells However significantly higher levels of CD4+Th1 Th2 and CD8+IFNγ+T cells were found in the pIRES/VP1/mGITRL group compared with control groups Our results demonstrate that a novel DNA vaccine expressing VP1 and mGITRL could effectively elicit both humoral and cellmediated immune responses against EV71 VP1 in mice Thus the mGITRL may be used as molecular adjuvant for EV71 DNA vaccineThis work was supported by the National Natural Science Foundation of China Grant No 31100648 81072453 Specialized Project for Clinical Medicine of Jiangsu Province Grant No BL2014065 Specialized Research Fund for the Doctoral Program of Higher Education Grant No 20133227110008 Health Department Foundation of Jiangsu Province Grant No Z201312 Science and technology support program Social Development of Zhenjiang Grant No SH2014039 Graduate Student Research and Innovation Program of Jiangsu Province Grant No CXZZ13 0700 KYLX 1074 Jiangsu Province “333” Project and Priority Academic Program Development of Jiangsu Higher Education Institutions
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