Authors: Shainaba A Saadhali Sameer Hassan Luke Elizabeth Hanna Uma Devi Ranganathan Vanaja Kumar
Publish Date: 2016/07/13
Volume: 22, Issue: 8, Pages: 180-
Abstract
Mycobacteriophages produce lysins that break down the host cell wall at the end of lytic cycle to release their progenies The ability to lyse mycobacterial cells makes the lysins significant Mycobacteriophage Che12 is the first reported temperate phage capable of infecting and lysogenising Mycobacterium tuberculosis Gp11 of Che12 was found to have Chitinase domain that serves as endolysin lysin A for Che12 Structure of gp11 was modeled and evaluated using Ramachandran plot in which 98 of the residues are in the favored and allowed regions Che12 lysin A was predicted to act on NAGNAMNAG molecules in the peptidoglycan of cell wall The tautomers of NAGNAMNAG molecule were generated and docked with lysin A The stability and binding affinity of lysin A – NAGNAMNAG tautomers were studied using molecular dynamics simulationsMs Shainaba A Saadhali is a recipient of Senior Research fellowship from Lady Tata Memorial Trust Mumbai India The authors would like to thank Dr P Velmurugan Head of the Department Department of Biophysics University of Madras Chennai India for his valuable suggestions The support and fund provided by National Institute for Research in Tuberculosis NIRT Chennai India is highly acknowledged The authors would like to thank Mr R Senthil Nathan NIRT Library for correcting the figures
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