Authors: T Ramakrishna M W Pandit
Publish Date: 1988/09/01
Volume: 13, Issue: 3, Pages: 215-222
Abstract
The concentrationdependent selfassociation of αchymotrypsin is known to be influenced by various factors including the presence of small molecules and autolysis products In this connection the effect of various amino acids on the selfassociation of αchymotrypsin has been studied as a point of interest by measuring the sedimentation coefficient of αchymotrypsin The influence of an amino acid is seen to be governed by the nature of its side chain Some amino acids do not affect the selfassociation of αchymotrypsin at all while some affect it moderately and some others considerably Functional groups such as the OH group of Ser or the phenolic ring of Tyr do not seem to influence selfassociation behaviour Based on these effects amino acids could be categorized into 3 groups Activity studies in the presence of amino acids indicate that the site of selfassociation and the activesite are probably mutually exclusive
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