Authors: S Anand J K Pal
Publish Date: 1997/06/01
Volume: 22, Issue: 3, Pages: 287-298
Abstract
The hemeregulated enkaryotic initiation factor2α eIF2α kinase also called the hemeregulated inhibitor HRI is a key regulator of protein synthesis in mammalian reticulocyte HRI is almost undetectable in blood samples of normal rabbits and it increases by 12–15fold in the reticulocytes of anemic rabbits In order to determine if such an increase in the quantity of HRI is gradual during anemia and if it could be an indicator of anemia we have carried out a detailed analysis on the expression of HRI and its eIF2α kinase activity in rabbit reticulocyte lysates during various stages of acetylphenylhydrazine APHinduced anemia In a 9day schedule of induction of anemia using an antiHRI monoclonal antibody HRI was detectable immediately after completion of fourth injection day 5 and it increased gradually during the entire period reaching its maximum 24fold on day 9 Furthermore when rabbits recovered from anemia due to individual response to the drug quantity of HRI decreased significantly Northern blot analysis results were similar to those of the Western blot The other parameters that are generally used to monitor anemia in human patients namely reticulocyte count haematocrit level and haemoglobin content although changed at the onset of anemia did not change significantly during its progression These results thus indicate that HRI could be a more appropriate and sensitive indicator of druginduced anemia
Keywords: