Authors: Guili Wang Gaowei Ren Xin Cui Zhitao Lu Yanpin Ma Ying Qi Yujing Huang Zhongyang Liu Zhengrong Sun Qiang Ruan
Publish Date: 2016/04/07
Volume: 41, Issue: 2, Pages: 173-182
Abstract
The interplay between the host and Human cytomegalovirus HCMV plays a pivotal role in the outcome of an infection HCMV growth in endothelial and epithelial cells requires expression of viral proteins UL128 UL130 and UL131 proteins UL128131 of which UL130 is the largest gene and the only one that is not interrupted by introns Mutation of the C terminus of the UL130 protein causes reduced tropism of endothelial cells EC However very few host factors have been identified that interact with the UL130 protein In this study HCMV UL130 protein was shown to directly interact with the human protein Snapin in human embryonic kidney HEK293 cells by Yeast twohybrid screening in vitro glutathione Stransferase GST pulldown and coimmunoprecipitation Additionally heterologous expression of protein UL130 revealed colocalization with Snapin in the cell membrane and cytoplasm of HEK293 cells using fluorescence confocal microscopy Furthermore decreasing the level of Snapin via specific small interfering RNAs decreased the number of viral DNA copies and titer in HCMVinfected U373S cells Taken together these results suggest that Snapin the pUL130 interacting protein has a role in modulating HCMV DNA synthesis
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