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Title of Journal: J Biosci

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Abbravation: Journal of Biosciences

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Springer-Verlag

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DOI

10.1007/0-306-48416-1_82

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0973-7138

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Efficient multisitedirected mutagenesis directly

Authors: Fengtao Luo Xiaolan Du Tujun Weng Xuan Wen Junlan Huang Lin Chen
Publish Date: 2012/09/29
Volume: 37, Issue: 1, Pages: 965-969
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Abstract

In this article the traditional multisitedirected mutagenesis method based on overlap extension PCR was improved specifically for complicated templates such as genomic sequence or complementary DNA This method was effectively applied for multisitedirected mutagenesis directly from mouse genomic DNA as well as for combination deletion or insertion of DNA fragments

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  15. Metabolic and molecular action of Trigonella foenum-graecum (fenugreek) and trace metals in experimental diabetic tissues
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