Journal Title
Title of Journal: J Comp Physiol B
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Abbravation: Journal of Comparative Physiology B
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Publisher
Springer-Verlag
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Authors: M EinickerLamas M M Morales K Miranda J GarciaAbreu A J F Oliveira F L S Silva M M Oliveira
Publish Date: 2003/07/22
Volume: 173, Issue: 7, Pages: 559-564
Abstract
Selective pressures from polluted environments have led to the development of resistance systems in aquatic organisms Using different techniques this study examined a cadmium defense mechanism of the freshwater unicellular protozoa Euglena gracilis and found it to be an efflux pump similar to the multidrug resistance Pglycoprotein Cd2+treated E gracilis were able to extrude Rhodamine 123 at 21 °C but not at 4 °C Furthermore verapamil a Pglycoprotein modulator partially blocked the efflux process at 21 °C and enhanced the Cd2+ toxic effects on these cells Western immunoblots of cell lysates using the antiPglycoprotein antibody JSB1 revealed a 120KDa protein which was expressed in high amounts on Cd2+exposed cells 74 above the control values Moreover cells treated with JSB1 became more sensitive to the harmful effects of cadmium showing a decreased survival rate Taken together these results suggest that a MDR phenotype has evolved in Euglena as one of the mechanisms for cadmium detoxificationThis work was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico CNPq and Fundação Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro FAPERJ The authors declare that the experiments comply with the current Brazilian laws
Keywords:
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Other Papers In This Journal:
- Springs, steroids, and slingshots: the roles of enhancers and constraints in animal movement
- Monoterpenes as inhibitors of digestive enzymes and counter-adaptations in a specialist avian herbivore
- P-glycoprotein-like protein contributes to cadmium resistance in Euglena gracilis
- Effects of melanocortin-4 receptor agonists and antagonists on expression of genes related to reproduction in spotted scat, Scatophagus argus
- Dehydration, rehydration, and overhydration alter patterns of gene expression in the Antarctic midge, Belgica antarctica
- Salt intake and regulation in two passerine nectar drinkers: white-bellied sunbirds and New Holland honeyeaters
- Daily rhythmicity and hibernation in the Anatolian ground squirrel under natural and laboratory conditions
- Evidence for proteins involved in prophenoloxidase cascade Eisenia fetida earthworms
- Effects of temperature, swimming speed and body mass on standard and active metabolic rate in vendace ( Coregonus albula )
- Intra- and extracellular osmotic regulation in the hololimnetic Caridea and Anomura: a phylogenetic perspective on the conquest of fresh water by the decapod Crustacea
- Lifetime- and caste-specific changes in flight metabolic rate and muscle biochemistry of honeybees, Apis mellifera
- Dietary assimilation and the digestive strategy of the omnivorous anomuran land crab Birgus latro (Coenobitidae)
- Activity affects intraspecific body-size scaling of metabolic rate in ectothermic animals
- Redistribution of blood within the body is important for thermoregulation in an ectothermic vertebrate ( Crocodylus porosus )
- Physiological properties of the gut lumen of terrestrial isopods (Isopoda: Oniscidea): adaptive to digesting lignocellulose?
- 65 Zn 2+ transport by lobster hepato-pancreatic baso-lateral membrane vesicles
- Innate immunity and testosterone rapidly respond to acute stress, but is corticosterone at the helm?
- Respiratory and digestive responses of postprandial Dungeness crabs, Cancer magister , and blue crabs, Callinectes sapidus , during hyposaline exposure
- The evolution of nitric oxide signalling in vertebrate blood vessels
- Brain thermal inertia, but no evidence for selective brain cooling, in free-ranging western grey kangaroos ( Macropus fuliginosus )
- Development of myoglobin concentration and acid buffering capacity in harp ( Pagophilus groenlandicus ) and hooded ( Cystophora cristata ) seals from birth to maturity
- Antioxidants in eggs of great tits Parus major from Chernobyl and hatching success
- Thermal acclimation to 4 or 10°C imparts minimal benefit on swimming performance in Atlantic cod ( Gadus morhua L.)
- A broader look at ammonia production, excretion, and transport in fish: a review of impacts of feeding and the environment
- Comparative patterns of adrenal activity in captive and wild Canada lynx ( Lynx canadensis )
- Glucocorticoid receptors are involved in the regulation of pulsatile urea excretion in toadfish
- Intestinal osmoregulatory acclimation and nitrogen metabolism in juveniles of the freshwater marble goby exposed to seawater
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