Journal Title
Title of Journal: J Comp Physiol B
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Abbravation: Journal of Comparative Physiology B
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Publisher
Springer-Verlag
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Authors: Petra Procházková Marcela Šilerová Benoit Stijlemans Marc Dieu Petr Halada Radka Josková Alain Beschin Patrick De Baetselier Martin Bilej
Publish Date: 2006/04/25
Volume: 176, Issue: 6, Pages: 581-587
Abstract
The prophenoloxidase cascade represents one of the most important defense mechanisms in many invertebrates Following the recognition of microbial saccharides by pattern recognition molecules proteinases cleave inactive prophenoloxidase to its active form phenoloxidase Phenoloxidase is a key enzyme responsible for the catalysis of the melanization reaction Final product melanin is involved in wound healing and immune responses Prophenoloxidase cascade has been widely described in arthropods data in other invertebrate groups are less frequent Here we show detectable phenoloxidase activity in 90kDa fraction of the coelomic fluid of earthworms Eisenia fetida Amino acid sequencing of peptides from the active fraction revealed a partial homology with invertebrate phenoloxidases and hemocyanins Moreover the level of phenoloxidase activity is lower and the activation slower as compared to other invertebratesThis work was supported by the Czech Science Foundation 310/04/0806 B500200613 Institutional Research Concept AVOZ50200510 and a bilateral international scientific and technological cooperation grant of the Ministry of the Flemish Community BOFBWS 03/06 and was performed within the frames of an Interuniversity Attraction Pole Program Authors are grateful to Prof Söderhäll Uppsala for providing probe containing the gene coding for PO in crayfish P leniusculus All experiments comply with the current laws of the Czech Republic and Belgium
Keywords:
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