Journal Title
Title of Journal: J Comp Physiol B
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Abbravation: Journal of Comparative Physiology B
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Publisher
Springer Berlin Heidelberg
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Authors: DongNeng Jiang JianTao Li YaXiong Tao HuaPu Chen SiPing Deng ChunHua Zhu GuangLi Li
Publish Date: 2017/02/14
Volume: 187, Issue: 4, Pages: 603-612
Abstract
Melanocortin4 receptor Mc4r function related to reproduction in fish has not been extensively investigated Here we report on gene expression changes by realtime PCR following treatment with Mc4r agonists and antagonists in the spotted scat Scatophagus argus Using in vitro incubated hypothalamus the Mc4r nonselective agonist NDPMSH Nle4 DPhe7αmelanocyte stimulating hormone 10−6 M and selective agonist THIQ N3R1 2 3 4Tetrahydroisoquinolinium3ylcarbonyl 1R14chlorobenzyl24cyclohexyl41H124triazol1ylmethyl piperidin1yl2oxoethylamine 10−7 M significantly increased the expression of gnrh Gonadotropin releasing hormone while the Mc4r nonselective antagonist SHU9119 AcNleAspHisDPhe/DNal2′ArgTrpLysNH2 10−6 M and selective antagonist Ipsen 5i compound 5i synthesized in Ipsen Research Laboratories 10−6 M significantly inhibited gnrh expression after 3 h of incubation In incubated pituitary tissue NDPMSH and THIQ significantly increased the expression of fshb Folliclestimulating hormone beta subunit and lhb Luteinizing hormone beta subunit while SHU9119 and Ipsen 5i significantly decreased fshb and lhb expression after 3 h of incubation During the in vivo experiment THIQ 1 mg/kg bw significantly increased gnrh expression in hypothalamic tissue as well as the fshb and lhb expression in pituitary tissue 12 h after abdominal injection Furthermore Ipsen 5i 1 mg/kg bw significantly inhibited gnrh expression in hypothalamic tissue as well as fshb and lhb gene expression in pituitary tissue 12 h after abdominal injection In summary Mc4r singling appears to stimulate gnrh expression in the hypothalamus thereby modulating the synthesis of Fsh and Lh in the pituitary In addition Mc4r also appears to directly regulate fshb and lhb levels in the pituitary in spotted scat Our study suggests that Mc4r through the hypothalamus and pituitary participates in reproductive regulation in fish
Keywords:
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