Mitochondrial DNA Restriction Map and Cytochrome c

Authors: Anna Kourti

Publish Date: 2006/09/15

Volume: 44, Issue: 7-8, Pages: 320-331

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Abstract

Corn stalk borer Sesamia nonagrioides Lefebvre Lepidoptera Noctuidae is among the most important insect pests of corn in the Mediterranean basin The mitochondrial DNA of this insect was purified and a restriction map was constructed The size of the mtDNA genome is 163 kb Genetic analysis of four corn stalk borer populations collected from Greece three populations and Spain one population was undertaken using DNA sequences of the mtDNA cytochrome oxidase CO I and II genes Sequencing of a 2079 bp region of these genes revealed 25 polymorphic sites among the populations Five molecular RFLP markers located in the mtDNA COI and COII genes were surveyed and two different haplotypes were detected Phylogenetic analysis based on COI/COII nucleotide sequences revealed genetic differentiation between samples and the results are discussed in relation to the geographic distribution of the corn stalk borer in two Mediterranean countries

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