Genome Characterization of a Breeding Line Derived

Authors: B P Keong J A Harikrishna

Publish Date: 2011/11/17

Volume: 50, Issue: 1-2, Pages: 135-145

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Abstract

A preliminary screening was conducted on BC3F1 and BC4F1 backcross families developed from crossing Oryza sativa MR219 and O rufipogon IRGC105491 Despite earlier results showing that O rufipogon alleles wild introgression contributed to both number of panicles qPPL2 and tillers qTPL2 at loci RM250 RM208 and RM48 in line A20 of the BC2F2 population we observed that wild introgression was lost at loci RM250 and RM208 but retained at locus RM48 in BC3F1 and BC4F1 Progeny tests conducted utilizing genotype and phenotype data on both BC4F1 and a reference population BC2F7 A20 line did not show significant differences between groups having the MR219 allele and wild introgression at locus RM48 This suggests that there is no additive and transgressive effect of wild introgression in the BC3F1 and BC4F1 generated The presence of wild introgression was largely due to gene contamination by crosspollination during field breeding practicesThe author would like to thank the Ministry of Science Technology and Innovation Malaysia MOSTI and the University of Malaya for funding and collaborators from Universiti Kebangsaan Malaysia MARDI Seberang Perai and University of Malaya for assistance

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