Journal Title
Title of Journal: Biochem Genet
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Abbravation: Biochemical Genetics
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Authors: Daisuke Seki Shinpei Obata Tsuyoshi Shirozu Takeshi Kitano Hisato Saitoh
Publish Date: 2010/06/13
Volume: 48, Issue: 9-10, Pages: 737-750
Abstract
At least four paralogs of the small ubiquitinrelated modifier SUMO exist in humans but there is limited information about SUMO paralogs from other vertebrate species We isolated the four cDNA encoding proteins similar to human SUMOs from the medaka fish Oryzias latipes OlSUMO1 OlSUMO2 OlSUMO3 and OlSUMO4 The amino acid sequences of OlSUMO2 3 and 4 are 89–94 identical but they share only 45 identity with OlSUMO1 Phylogenetic analysis transient expression of OlSUMOs in cultured cells and in vitro binding of OlSUMOs with two SUMOinteracting proteins demonstrated that the medaka SUMO paralogs can be grouped into two subfamilies OlSUMO1 and OlSUMO2/3/4 Furthermore this is the first report of all four OlSUMO transcripts being expressed in medaka embryos implying that they have a role in fish development This study will improve understanding of the relationship between structural and functional diversity of SUMO paralogs during vertebrate evolutionWe thank Ms Maki Kuroki for help with extraction of RNA from medaka embryos We appreciate the helpful discussions with the members of the Saitoh Laboratory This study was supported by grants to Hisato Saitoh from the Naito Foundation the Novartis Foundation the Ministry of Education Culture Sport Science and Technology MEXT and the Japan Society for the Promotion of Science JSPS Daisuke Seki is a Junior Research Associate of the global COE Program at the Global Initiative Center for Pulsed Power Engineering
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