Triploid origin of the gibel carp as revealed by 5

Authors: H P Zhu D M Ma J F Gui

Publish Date: 2006/11/22

Volume: 14, Issue: 7, Pages: 767-776

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Abstract

5S ribosomal DNA rDNA was isolated and sequenced from the gibel carp Carassius auratus gibelio with 162 chromosomes and crucian carp Carassius auratus with 100 chromosomes and fluorescent probes for chromosome localization were prepared to ascertain the ploidy origin and evolutionary relationship between the two species Using fluorescence insitu hybridization FISH major 5S rDNA signals were localized to the short arms of three subtelocentric chromosomes in the gibel carp and to the short arms of two subtelocentrics in the crucian carp In addition some minor signals were detected on other chromosomes of both species Simultaneously six chromosomes were microdissected from the gibel carp metaphase spreads using glass needles and the isolated chromosomes were amplified in vitro by degenerate oligonucleotide primedpolymerase chain reaction DOPPCR Significantly when the DOPPCRgenerated probes prepared from each single chromosome were hybridized three samesized chromosomes were painted in each gibel carp metaphase whereas only two painted chromosomes were observed in each crucian carp metaphase spread The data indicate that gibel carp is of triploid origin in comparison with diploid crucian carp

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