Dose dependent inhibitory effect of dietary carawa

Authors: Kumaraswami Deeptha Muthaiyan Kamaleeswari Murugan Sengottuvelan Namasivayam Nalini

Publish Date: 2006/03/31

Volume: 24, Issue: 6, Pages: 479-488

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Abstract

Colon cancer has become one of the major causes of cancer mortality We determined the effect of caraway Carum carvi L on the development of aberrant crypt foci ACF and modulation of fecal bacterial enzyme activities in 12dimethylhydrazine DMHinduced experimental rat colon carcinogenesis Male Wistar albino rats were divided into six groups and all the animals were fed 158 peanut oil making a total of 20 fat in the diet Group 1 served as control and group 2 animals received 90 mg/kg body weight caraway po daily for 15 weeks To induce ACF DMH 20 mg/kg body weight was injected subcutaneously once a week for the first four weeks groups 3–6 In addition caraway was administered at the dose of 30 60 and 90 mg/kg body weight everyday orally for the entire period of 15 weeks groups 4–6 First we analyzed ACF number incidence multiplicity and its distribution along the colon in all experimental groups at the end of 15 weeks Subsequently we also assayed the fecal bacterial enzyme activities ACF formation and the fecal bacterial enzyme activities were found to be significantly high in DMHalone treated group as compared to control group Caraway supplementation at three different doses significantly suppressed ACF development bacterial enzyme activities and modulated oxidative stress significantly as compared to the unsupplemented DMHtreated group Results of our present study indicate that dietary caraway markedly inhibited DMHinduced colon carcinogenesis and the optimal dose of 60 mg/kg body weight was more effective than the other two doses

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