Authors: Kagayaki Morishima Ichiro Nakayama Katsutoshi Arai
Publish Date: 2007/07/04
Volume: 132, Issue: 3, Pages: 227-241
Abstract
In the present study the first genetic linkage map of the loach Misgurnus anguillicaudatus was constructed with 164 microsatellite markers and a color locus and it included 155 newly developed markers A total of 159 microsatellite markers and a color locus were mapped in 27 linkage groups LGs The female map covered 7845 cM with 153 microsatellite markers and a color locus whereas the male map covered 6622 cM with 119 microsatellite markers The centromeric position in each LG was estimated by markercentromere mapping based on halftetrad analysis In 4 LGs LG2 LG3 LG4 and LG5 the centromere was estimated at the intermediate region In LG1 LG11 and LG12 the centromere was estimated to shift from the subintermediate region to the end telomeric The number of these LGs 7 was identical to the collective number of biarm metacentric 5 and submetacentric chromosome 2 of the haploid chromosome set n = 5 of the loach In the other LGs the position of the centromere was estimated at the end or outside These results indicate satisfactory compliance between the linkage map and the chromosome set Our map would cover approximately almost the entire loach genome because most markers were successfully mappedWe thank Prof Heisuke Nakagawa and Dr Tetsuya Umino Laboratory of Aquaculture Graduate School of Biosphere Science Hiroshima University for their advice on the present study This study was supported in part by a GrantinAid for 21stCentury COE Center of Excellence Program from the Ministry of Education Culture Sports Science and Technology MEXT and by Scientific Research BNo18380108 from the Japanese Society for the Promotion of Science JSPS awarded to Katsutoshi Arai
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