Authors: Gang Li Wei Hu Rui Qin Huajun Jin Guangxuan Tan Lili Zhu Guangcun He
Publish Date: 2007/11/04
Volume: 134, Issue: 2, Pages: 169-180
Abstract
Wild rice is a valuable resource for the genetic improvement of cultivated rice Oryza sativa L AA genome Molecular markers are important tools for monitoring gene introgression from wild rice into cultivated rice In this study Simple sequence repeat SSR markers were used to analyze interspecific hybrids of O sativa–O officinalis CC genome the backcrossing progenies and the parent plants Results showed that most of the SSR primers 335 out of 396 846 developed in cultivated rice successfully amplified products from DNA samples of wild rice O officinalis The polymorphism ratio of SSR bands between O sativa and O officinalis was as high as 939 indicating differences between the two species with respect to SSRs When the SSR markers were applied in the interspecific hybrids only a portion of SSR primers amplified O officinalisspecific bands in the F1 hybrid 525 BC1 525 and MAALs 370 a number of the bands disappeared Of the 124 SSR loci that detected officinalisspecific bands in MAAL plants 96 774 showed synteny between the A and Cgenomes and 20 161 showed duplication in the Cgenome Sequencing analysis revealed that indels substitution and duplication contribute to the diversity of SSR loci between the genomes of O sativa and O officinalis
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