Journal Title
Title of Journal: Genetica
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Publisher
Springer International Publishing
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Authors: Solene Janique Wantana Sriratanasak Kulchana Ketsuwan Jirapong Jairin Ekgachai Jeratthitikul
Publish Date: 2017/01/09
Volume: 145, Issue: 1, Pages: 37-49
Abstract
The Asian rice gall midge RGM Orseolia oryzae Wood Mason Diptera Cecidomyiidae is a major pest of rice leading to yield losses in Thailand and many Asian countries Despite an increasing number of reported midge outbreaks and the presence of many susceptible rice varieties only a few studies have focused on the genetic variation of the midges Therefore we analyzed the phylogeography among Thai RGM populations covering north northeast and central Thailand Two mitochondrial DNA genes cytochrome C oxidase I COI and 12S and a noncoding repeat region RR situated just before COI were amplified Overall the haplotype diversity for COI and 12S genes of the Thai population was high but the nucleotide diversity was quite low Altogether the phylogenetic tree and pairwise F st values indicated that Thai RGM populations recently expanded and were homogeneously distributed throughout the country except for some populations in the north which most likely became recently isolated from the main population Two noncoding repeat motifs that were recently observed in the mitogenome of RGM in India were absent in Thai populations and replaced by an 89 bp noncoding sequence Tandem nucleotide repeats of the sequence TA were also observed The repeat copy number varied from 2 to 11 and was not correlated with geographical repartition of the midge Finally COI barcoding divergence between Indian and Thai populations was high 63 in average giving insights into the potential existence of an RGM species complex in AsiaThis research project was supported by Mahidol University under the Talent Management Program and Faculty of Science Mahidol University to EJ We also would like to give our regards to Dr Sangvorn Kitthawee and Dr Jenjit Khudamrongsawat for comments as well as Dr James Rudge and Stephan Schreier for comments and grammar checking
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