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Title of Journal: Protein J

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Abbravation: The Protein Journal

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Springer US

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1573-4943

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Polyclonal Antibodies from Hen Egg Yolk IgY with

Authors: Xianghui Ma Shuqing Sun Xiangming Ma Ting Li Jie Meng Jungang Fan
Publish Date: 2007/08/31
Volume: 26, Issue: 7, Pages: 499-
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Abstract

Polyclonal catalytic antibodies abzymes play an important role in immunology research In this study we report polyclonal antibodies IgYs isolated from chicken egg yolk with hydrolysis activity for the first time The IgYs were raised in hens using HNPBV 4hydroxy naphthalen2yloxy phosphoryl butanoic acid attached to BSA Bovine serum albumin as an immunogen AntiHNPBVBSA IgYs were isolated from yolks of the eggs laid using a twostep salt precipitation and onestep gel filtration protocol NA naphthalen2yl acetate was selected as the substrate and the hydrolysis reaction of the IgYs for it was examined The result reveals that the rate of the hydrolysis reaction is higher K cat/K uncat∼2 × 104 The purified IgYs were digested with pepsin and the smaller fragment Fab′ with specific antigen binding properties was produced The research indicates that the enzymatic properties of Fab′ are similar to IgYs The catalytic activity of the IgYs was further determined by measuring the rate of hydrolysis of NA in the presence of inhibitor These findings show that chicken egg is an excellent donor for polyclonal catalytic antibodies

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  2. The Use of the Free Metal – Temperature ‘Phase Diagrams’ for Studies of Single Site Metal Binding Proteins
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  4. Effects of Pulsed Electric Field (PEF) Treatment on Enhancing Activity and Conformation of α-Amylase
  5. Isolation, Purification and Characterization of Two Laccases from Carrot ( Daucus carota L.) and Their Response to Abiotic and Metal Ions Stresses
  6. Biological and Structural Characterization of Crotoxin and New Isoform of Crotoxin B PLA 2 (F6a) from Crotalus durissus collilineatus Snake Venom
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  9. Biophysical Analysis of the N-Terminal Domain from the Human Protein Phosphatase 1 Nuclear Targeting Subunit PNUTS Suggests an Extended Transcription Factor TFIIS-Like Fold
  10. Improved Protein Overexpression and Purification Strategies for Structural Studies of Cyanobacterial Metal-Responsive Transcription Factor, SmtB from Marine Synechococcus sp. PCC 7002
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  13. Nucleotide Binding to the Human Multidrug Resistance Protein 3, MRP3
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