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Title of Journal: Protein J

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Abbravation: The Protein Journal

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Springer US

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DOI

10.1002/chin.198736160

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1573-4943

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Effects of Pulsed Electric Field PEF Treatment o

Authors: Meiling Tian Ting Fang Muying Du Fusheng Zhang
Publish Date: 2016/02/20
Volume: 35, Issue: 2, Pages: 154-162
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Abstract

To explore an efficient safe and speedy application of pulsed electric field PEF technology for enzymatic modification effects of PEF treatment on the enzymatic activity property and kinetic parameters of αamylase were investigated Conformational transitions were also studied with the aid of circular dichroism CD and fluorescence spectra The maximum enzymatic activity of αamylase was obtained under 15 kV/cm electric field intensity and 100 mL/min flow velocity PEF treatment in which the enzymatic activity increased by 2213 ± 114  compared with control The activation effect could last for 18 h at 4 °C PEF treatment could widen the range of optimum temperature for αamylase however it barely exerted any effect on the optimum pH On the other hand αamylase treated by PEF showed an increase of V max t1/2 and ΔG whereas a decrease of K m and k were observed Furthermore it can be observed from fluorescence and CD spectra that PEF treatment had increased the number of amino acid residues especially that of tryptophan on αamylase surface with enhanced αhelices by 3476  and decreased random coil by 1204  on αamylase when compared with that of untreated These changes in structure had positive effect on enhancing αamylase activity and property

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