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Title of Journal: Protein J

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Abbravation: The Protein Journal

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Springer US

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10.1007/s003300000566

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1573-4943

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Influence of Polyols on the Stability and Kinetic

Authors: Gangadhara Parigi Ramesh Kumar Vishweshwaraiah Prakash
Publish Date: 2008/11/20
Volume: 27, Issue: 7-8, Pages: 440-449
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Abstract

Invertase βdfructofuranoside fructohydrolaseEC 32126 is a sucrose hydrolyzing enzyme found in microbial plant and animal sources Invertase from Candida utilis is a dimeric glycoprotein composed of two identical monomer subunits with an apparent molecular mass of 150 kDa We investigated the mechanism of stabilization of invertase with polyols glycerol xylitol and sorbitol Activity thermodynamic and kinetic measurements of invertase were performed as a function of polyol concentration and showed that polyols act as very effective stabilizing agents The result from the solventinvertase interaction shows preferential exclusion of the polyols from the protein domain leading to preferential hydration of protein Apparent thermal denaturation temperature of the protein T m rose from 75 °C to a maximum of 85 °C in 30 glycerol The stabilization has been attributed to the preferential hydration of the enzyme

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  1. Characterization and Expression of a GDSL-Like Lipase Gene from Brassica napus in Nicotiana benthamiana
  2. The Use of the Free Metal – Temperature ‘Phase Diagrams’ for Studies of Single Site Metal Binding Proteins
  3. Effects of Furanocoumarins from Apiaceous Vegetables on the Catalytic Activity of Recombinant Human Cytochrome P-450 1A2
  4. Effects of Pulsed Electric Field (PEF) Treatment on Enhancing Activity and Conformation of α-Amylase
  5. Isolation, Purification and Characterization of Two Laccases from Carrot ( Daucus carota L.) and Their Response to Abiotic and Metal Ions Stresses
  6. Biological and Structural Characterization of Crotoxin and New Isoform of Crotoxin B PLA 2 (F6a) from Crotalus durissus collilineatus Snake Venom
  7. Conformational Change in the Active Site of Streptococcal Unsaturated Glucuronyl Hydrolase Through Site-Directed Mutagenesis at Asp-115
  8. Molecular Modeling of the Plasmodium falciparum Pre-mRNA Splicing and Nuclear Export Factor PfU52
  9. Biophysical Analysis of the N-Terminal Domain from the Human Protein Phosphatase 1 Nuclear Targeting Subunit PNUTS Suggests an Extended Transcription Factor TFIIS-Like Fold
  10. Improved Protein Overexpression and Purification Strategies for Structural Studies of Cyanobacterial Metal-Responsive Transcription Factor, SmtB from Marine Synechococcus sp. PCC 7002
  11. Polyclonal Antibodies from Hen Egg Yolk (IgY) with Hydrolysis Activity
  12. The Role of Strong Electrostatic Interactions at the Dimer Interface of Human Glutathione Synthetase
  13. Nucleotide Binding to the Human Multidrug Resistance Protein 3, MRP3
  14. Isolation and Characterization of a Kunitz-Type Trypsin Inhibitor with Antiproliferative Activity from Gymnocladus Chinensis (Yunnan Bean) Seeds
  15. The Estimation of GC Repeats in Promoter P1 of IGF - 1 Gene and Their Influence on IGF-1 Plasma Levels in Stable Angina Patients
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