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Title of Journal: Protein J

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Abbravation: The Protein Journal

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Springer US

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DOI

10.1002/asna.19402700403

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1573-4943

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Isolation and Characterization of a KunitzType Tr

Authors: M J Zhu G Q Zhang H X Wang T B Ng
Publish Date: 2011/04/06
Volume: 30, Issue: 4, Pages: 240-246
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Abstract

A 20kDa Kunitztype trypsin inhibitor was isolated from Gymnocladus chinensis Yunnan bean seeds The isolation procedure involved ion exchange chromatography on diethylaminoethyl cellulose DEAEcellulose affinity chromatography on Affigel blue gel ion exchange chromatography on sulfopropyl sepharose SPsepharose and gel filtration by FPLC on Superdex 75 The trypsin inhibitor was adsorbed on DEAEcellulose unadsorbed on Affigel blue gel and adsorbed on SPSepharose It dosedependently inhibited trypsin with an IC50 value of 04 μM Dithiothreitol reduced its trypsin inhibitory activity suggesting that an intact disulfide bond is indispensable to the activity It suppressed methyl3H thymidine incorporation by leukemia L1210 cells and lymphoma MBL2 cells with an IC50 value of 47 and 94 μM respectively There was no effect on human immunodeficiency virus41 reverse transcriptase activity and fungal growth when the trypsin inhibitor was tested up to 100 μM

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  1. Characterization and Expression of a GDSL-Like Lipase Gene from Brassica napus in Nicotiana benthamiana
  2. The Use of the Free Metal – Temperature ‘Phase Diagrams’ for Studies of Single Site Metal Binding Proteins
  3. Effects of Furanocoumarins from Apiaceous Vegetables on the Catalytic Activity of Recombinant Human Cytochrome P-450 1A2
  4. Effects of Pulsed Electric Field (PEF) Treatment on Enhancing Activity and Conformation of α-Amylase
  5. Isolation, Purification and Characterization of Two Laccases from Carrot ( Daucus carota L.) and Their Response to Abiotic and Metal Ions Stresses
  6. Biological and Structural Characterization of Crotoxin and New Isoform of Crotoxin B PLA 2 (F6a) from Crotalus durissus collilineatus Snake Venom
  7. Conformational Change in the Active Site of Streptococcal Unsaturated Glucuronyl Hydrolase Through Site-Directed Mutagenesis at Asp-115
  8. Molecular Modeling of the Plasmodium falciparum Pre-mRNA Splicing and Nuclear Export Factor PfU52
  9. Biophysical Analysis of the N-Terminal Domain from the Human Protein Phosphatase 1 Nuclear Targeting Subunit PNUTS Suggests an Extended Transcription Factor TFIIS-Like Fold
  10. Improved Protein Overexpression and Purification Strategies for Structural Studies of Cyanobacterial Metal-Responsive Transcription Factor, SmtB from Marine Synechococcus sp. PCC 7002
  11. Influence of Polyols on the Stability and Kinetic Parameters of Invertase from Candida utilis : Correlation with the Conformational Stability and Activity
  12. Polyclonal Antibodies from Hen Egg Yolk (IgY) with Hydrolysis Activity
  13. The Role of Strong Electrostatic Interactions at the Dimer Interface of Human Glutathione Synthetase
  14. Nucleotide Binding to the Human Multidrug Resistance Protein 3, MRP3
  15. The Estimation of GC Repeats in Promoter P1 of IGF - 1 Gene and Their Influence on IGF-1 Plasma Levels in Stable Angina Patients
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