Authors: Malin Bomberg Sari Timonen
Publish Date: 2007/03/06
Volume: 54, Issue: 3, Pages: 406-416
Abstract
Archaeal 16S rRNA gene sequences have been found in a variety of moderatetemperature habitats including soil and rhizospheres In this study the differences of archaeal communities associated with Scots pine Pinus sylvestris L short roots different types of mycorrhizospheric compartments and uncolonized boreal forest humus were tested by direct DNA extraction polymerase chain reaction–denaturing gradient gel electrophoresis PCR–DGGE and sequencing The results indicated that mycorrhizal colonization of Scots pine roots substantially influence the archaeal community of pine rhizospheres Colonization of short roots by most mycorrhizal fungi tested increased both archaeal frequency and diversity Most of the archaeal sequences encountered in mycorrhizas belonged to the phylum Euryarchaeota order of Halobacteriales The difference in archaeal diversity between the mycorrhizospheric compartments and humus was profound Most compartments with fungal components contained euryarchaeotal 16S rRNA gene sequences whereas a high diversity of crenarchaeotal sequences and no euryarchaeotal sequences were found in forest humus outside mycorrhizospheresThe Maj and Tor Nessling Foundation Svenska Kulturfonden University of Helsinki and Academy of Finland are thanked for financial support Dr Paula Kristo is thanked for her expert help in sequencing and Dr Jodie Painter for her constructive comments on the manuscript We also wish to warmly thank Professor Jari Valkonen for providing us with excellent working environment both in atmosphere and equipment
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