Authors: XiaoMing Lu PengZhen Lu
Publish Date: 2014/01/30
Volume: 67, Issue: 3, Pages: 612-623
Abstract
454 Pyrosequencing was applied to examine bacterial communities in sediment samples collected from a river receiving effluent discharge from rural domestic sewage RDS and various factories including a tannery TNS clothing plant CTS and button factory BTS respectively For each sample 4510 effective sequences were selected and utilized to do the bacterial diversity and abundance analysis respectively In total 1288 2036 1800 and 2150 operational taxonomic units were obtained at 3 distance cutoff in TNS CTS BTS and RDS respectively Bacterial phylotype richness in RDS was higher than the other samples and TNS had the least richness The most predominant class in the TNS CTS and BTS samples is Betaproteobacteria Cyanobacteria no rank is the most predominant one in the RDS sample Circa 31 sequences in TNS were affiliated with the Rhodocyclales order In the four samples Aeromonas Arcobacter Clostridium Legionella Leptospira Mycobacterium Pseudomonas and Treponema genera containing pathogenic bacteria were detected Characterization of bacterial communities in sediments from various downstream branches indicated that distinct wastewater effluents have similar potential to reduce the natural variability in river ecosystems and contribute to the river biotic homogenizationThis work was supported by the Science and Technology Innovation Project of Wenzhou City No C201200503 the Foundation of Zhejiang Educational Committee No Y201224611 the China Postdoctoral Science Foundation No 2013 M541647 and the Jiangsu Planned Projects for Postdoctoral Research Funds No 1302130C
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