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Title of Journal: Microb Ecol

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Abbravation: Microbial Ecology

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Springer US

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ISSN

1432-184X

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Morphological and Phylogenetic Analysis of Emphas

Authors: Khosrow Chehri Baharuddin Salleh Latiffah Zakaria
Publish Date: 2014/09/20
Volume: 69, Issue: 3, Pages: 457-471
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Abstract

Members of Fusarium solani species complex FSSC have been known as plant animal and human pathogens Nevertheless the taxonomic status of such an important group of fungi is still very confusing and many new species as well as lineages have been elucidated recently Unfortunately most of the new taxa came from temperate and subtropical regions Therefore the objectives of the present study were to identify strains of FSSC recovered from different sources in Malaysia In the present study 55 strains belonging to the FSSC were examined and phylogenetically analyzed on the basis of internal transcribed spacer ITS regions and partial translation elongation factor1 TEF1α sequences Based on morphological features a total of 55 strains were selected for molecular studies Based on morphological features the strains were classified into four described Fusarium species namely Fusarium keratoplasticum Fusarium falciforme FSSC 5 and Fusarium cf ensiforme and one unknown phylogenetic species was introduced Although the data obtained from morphological and molecular studies sufficiently supported each other the phylogenetic trees based on ITS and TEF1α dataset clearly distinguished closely related species and distinctly separated all morphological taxa All members of FSSC in this research were reported for the first time for Malaysian mycofloraMost members of the Fusarium solani species complex FSSC are frequently isolated from soils and act as decomposers but some are putative parasites on plants insects humans and animals 1 2 3 4 Wollenweber and Reinking 5 divided members of F solani into two sections of Ventricosum and Martiella Snyder and Hansen 6 represented FSSC as a complex species in the Martiella section 1 7 8 Seven mating populations MPI–VII were determined for F solani 9 10 11 12 Members of FSSC mating populations MPI MPV and MPVI were placed in distinct groups by phylogenetic analysis 13 Molecular phylogenetic demonstrated that FSSC MPI and MPV as F solani f sp cucurbitae races 1 and 2 respectively were polyphyletic 14 15Because of the significant role of members of the FSSC in clinical infection and complication in their determination molecular identification strategies have been emphasized for their identification in the last 20 years 2 3 Phylogenetic analysis by 28S ribosomal DNA internal transcribed spacer ITS regions and tef1 gene sequences revealed high variability within members of the FSSC and all 55 diagnosable species were divided into three clades termed clades 1 2 and 3 3 15 16 17 Members of clade 1 comprised two known species Fusarium illudens and Nectria plagianthi from New Zealand Members of clade 2 included a number of important pathogens that cause sudden death syndrome SDS of soybean 18 19 20 Nalim et al 17 used molecular phylogeny to show that members of FSSC in clade 2 are paraphyleticMolecular phylogeny showed diverse phylogenetic affinities among members of clade 3 This group encompassed many species that are important in agricultural crops and medicine 15 Members of clade 3 are the most common group of fusaria associated with plant diseases and human infections Members of Fusarium falciforme FSSC 3 + 4 and Fusarium keratoplasticum as most haplotypediverse species were placed among clade 3 2 3 15 21 22 Several studies to date have revealed different phylogenetic species within this important evolutionary clade though little work has been done to improve the taxonomy and therefore correct identification of species as one of the prerequisites in any disease control program has become more challenging Although the taxonomic status of FSSC from all over the world is being revised and a strong connection has been revealed among strains recovered from humans insects and plants 2 17 21 22 23 24 until today no attempt has been made to classify members of the FSSC in tropical Southeast Asia particularly Malaysia Therefore the objectives of this study were to identify strains of FSSC recovered from different substrates in Malaysia by using morphological characteristics and sequencing of ITS region and translation elongation factor1α TEF1α to determine genetic relationship among them


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  1. Frequency and Diversity of Nitrate Reductase Genes among Nitrate-Dissimilating Pseudomonas in the Rhizosphere of Perennial Grasses Grown in Field Conditions
  2. Characterization of Bacterial Communities in Sediments Receiving Various Wastewater Effluents with High-Throughput Sequencing Analysis
  3. Bacterial Community Dynamics during Bioremediation of Diesel Oil-Contaminated Antarctic Soil
  4. Assessing the Contamination Potential of Freshly Extracted Escherichia coli Biofilm Cells by Impedancemetry
  5. Denitrifying Bacterial Community Composition Changes Associated with Stages of Denitrification in Oxygen Minimum Zones
  6. Is There a Size Limit for Cosmopolitan Distribution in Free-Living Microorganisms? A Biogeographical Analysis of Testate Amoebae from Polar Areas
  7. Vegetation Affects the Relative Abundances of Dominant Soil Bacterial Taxa and Soil Respiration Rates in an Upland Grassland Soil
  8. Epilithic Algae Distribution Along a Chemical Gradient in a Naturally Acidic River, Río Agrio (Patagonia, Argentina)
  9. Distribution of Cren- and Euryarchaeota in Scots Pine Mycorrhizospheres and Boreal Forest Humus
  10. Feasibility of Removing Surface Deposits on Stone Using Biological and Chemical Remediation Methods
  11. Molecular Characterization and Geological Microenvironment of a Microbial Community Inhabiting Weathered Receding Shale Cliffs
  12. Previously Undescribed Plasmids Recovered from Activated Sludge Confer Tetracycline Resistance and Phenotypic Changes to Acinetobacter oleivorans DR1
  13. Culturable Airborne Bacteria in Outdoor Environments in Beijing, China
  14. Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by Sphingomonas paucimobilis Strain 20006FA
  15. Ecological Characterisation of the Colonic Microbiota in Arctic and Sub-Arctic Seals
  16. Isolation and Identification of the Microbiota of Danish Farmhouse and Industrially Produced Surface-Ripened Cheeses
  17. Effects of Experimental Lead Pollution on the Microbial Communities Associated with Sphagnum fallax (Bryophyta)
  18. Habitat Heterogeneity and Associated Microbial Community Structure in a Small-Scale Floodplain Hyporheic Flow Path
  19. Abundance of Class 1 Integron-Integrase and Sulfonamide Resistance Genes in River Water and Sediment Is Affected by Anthropogenic Pressure and Environmental Factors
  20. Patterns of Multi-Antibiotic-Resistant Escherichia Coli from Streams with No History of Antimicrobial Inputs
  21. Does the Reproductive Strategy Affect the Transmission and Genetic Diversity of Bionts in Cyanolichens? A Case Study Using Two Closely Related Species
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  23. Strong Indirect Effects of a Submersed Aquatic Macrophyte, Vallisneria americana , on Bacterioplankton Densities in a Mesotrophic Lake
  24. Erratum to: Influence of Deglaciation on Microbial Communities in Marine Sediments off the Coast of Svalbard, Arctic Circle
  25. Microbial Characterization during the Early Habitation of the International Space Station
  26. 16S rRNA and As-Related Functional Diversity: Contrasting Fingerprints in Arsenic-Rich Sediments from an Acid Mine Drainage
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  28. Methanogen Colonisation Does Not Significantly Alter Acetogen Diversity in Lambs Isolated 17 h After Birth and Raised Aseptically
  29. Changes in Communities of Fusarium and Arbuscular Mycorrhizal Fungi as Related to Different Asparagus Cultural Factors
  30. Pond Sediment Magnetite Grains Show a Distinctive Microbial Community
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  33. Amazonian Dark Earth and Plant Species from the Amazon Region Contribute to Shape Rhizosphere Bacterial Communities
  34. Eggshell Bacterial Load Is Related to Antimicrobial Properties of Feathers Lining Barn Swallow Nests
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