Authors: Lichi Shi Xiaohu Peng Mumdooh A M Ahmed Dale Edwards Leonid S Brown Vladimir Ladizhansky
Publish Date: 2008/04/11
Volume: 41, Issue: 1, Pages: 9-15
Abstract
We describe a simple protocol to achieve homonuclear Jdecoupling in the indirect dimensions of multidimensional experiments and to enhance spectral resolution of the backbone Cα carbons in the 3D NCACX experiment In the proposed protocol the refocusing of the Cα–CO homonuclear Jcouplings is achieved by applying an offresonance selective π pulse to the CO spectral region in the middle of Cα chemical shift evolution As is commonly used in solution NMR a compensatory echo period is used to refocus the unwanted chemical shift evolution of Cα spins which takes place during the offresonance selective pulse The experiments were carried out on the β1 immunoglobulin binding domain of protein G GB1 In GB1 such implementation results in significantly reduced line widths and leads to an overall sensitivity enhancementThis research was supported by the University of Guelph startup funds to VL the Natural Sciences and Engineering Research Council of Canada DG250202 to LSB and Grants RG29848004 to VL the Canada Foundation for Innovation and the Ontario Innovation Trust VL holds Canada Research Chair in Biophysics and is a recipient of an Early Researcher Award from the Ontario Ministry of Research and Innovation LS is a recipient of the Ontario Graduate Scholarship MA is a recipient of a Doctoral Studentship from the Ministry of Higher Education and Scientific Research of Egypt
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