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Title of Journal: J Biomol NMR

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Abbravation: Journal of Biomolecular NMR

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Springer Netherlands

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DOI

10.1016/0169-8141(92)90127-l

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1573-5001

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Outandback Superscript13/SuperscriptC–Super

Authors: Emeline BarbetMassin Andrew J Pell Kristaps Jaudzems W Trent Franks Joren S Retel Svetlana Kotelovica Inara Akopjana Kaspars Tars Lyndon Emsley Hartmut Oschkinat Anne Lesage Guido Pintacuda
Publish Date: 2013/06/29
Volume: 56, Issue: 4, Pages: 379-386
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Abstract

We present here 1Hdetected tripleresonance H/N/C experiments that incorporate CO–CA and CA–CB outandback scalartransfer blocks optimized for robust resonance assignment in biosolids under ultrafast magicangle spinning MAS The first experiment HCOCACONH yields 1Hdetected interresidue correlations in which we record the chemical shifts of the CA spins in the first indirect dimension while during the scalartransfer delays the coherences are present only on the longerlived CO spins The second experiment HCACBCANH correlates the sidechain CB chemical shifts with the NH of the same residue These high sensitivity experiments are demonstrated on both fullyprotonated and 100 HN backprotonated perdeuterated microcrystalline samples of Acinetobacter phage 205 AP205 capsids at 60 kHz MAS

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  13. S3EPY: a Sparky extension for determination of small scalar couplings from spin-state-selective excitation NMR experiments
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