Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: J Biomol NMR

Search In Journal Title:

Abbravation: Journal of Biomolecular NMR

Search In Journal Abbravation:

Publisher

Springer Netherlands

Search In Publisher:

DOI

10.1007/bf01136431

Search In DOI:

ISSN

1573-5001

Search In ISSN:
Search In Title Of Papers:

Selective Superscript1/SuperscriptHSuperscri

Authors: Chenyun Guo Vitali Tugarinov
Publish Date: 2009/12/03
Volume: 46, Issue: 2, Pages: 127-133
PDF Link

Abstract

Methyl 13CHD2 isotopomers of all methylcontaining aminoacids can be observed in residually protonated samples of large proteins obtained from U13C1Hglucose/D2Obased bacterial media with sensitivity sufficient for a number of NMR applications Selective detection of some subsets of methyl groups Alaβ Thrγ2 is possible using simple ‘outandback’ NMR methodology Such selective methyldetected ‘outandback’ NMR experiments allow complete assignments of threonine γ2 methyls in residually protonated U13C1Hglucose/D2Oderived samples of an 82kDa enzyme Malate Synthase G U13C1Hglucose/D2Oderived protein samples are relatively inexpensive and are usually available at very early stages of any NMR study of highmolecularweight systems

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Detection of nanosecond time scale side-chain jumps in a protein dissolved in water/glycerol solvent
  2. Statistical removal of background signals from high-throughput 1 H NMR line-broadening ligand-affinity screens
  3. Peak picking multidimensional NMR spectra with the contour geometry based algorithm CYPICK
  4. HNHC: a triple resonance experiment for correlating the H2, N1(N3) and C2 resonances in adenine nucleobases of 13 C-, 15 N-labeled RNA oligonucleotides
  5. NOESY-WaterControl: a new NOESY sequence for the observation of under-water protein resonances
  6. Insights into furanose solution conformations: beyond the two-state model
  7. Fast methionine-based solution structure determination of calcium-calmodulin complexes
  8. A procedure to validate and correct the 13 C chemical shift calibration of RNA datasets
  9. Complete dissociation of the HIV-1 gp41 ectodomain and membrane proximal regions upon phospholipid binding
  10. NMR solution structure of the acylphosphatase from Escherichia coli
  11. A rigid disulfide-linked nitroxide side chain simplifies the quantitative analysis of PRE data
  12. S3EPY: a Sparky extension for determination of small scalar couplings from spin-state-selective excitation NMR experiments
  13. Improved NMR experiments with 13 C-isotropic mixing for assignment of aromatic and aliphatic side chains in labeled proteins
  14. Out-and-back 13 C– 13 C scalar transfers in protein resonance assignment by proton-detected solid-state NMR under ultra-fast MAS
  15. Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization
  16. A probe to monitor performance of 15 N longitudinal relaxation experiments for proteins in solution
  17. Optimization of NMR spectroscopy of encapsulated proteins dissolved in low viscosity fluids
  18. Use of quantitative 1 H NMR chemical shift changes for ligand docking into barnase
  19. Resolution enhancement by homonuclear J-decoupling: application to three-dimensional solid-state magic angle spinning NMR spectroscopy
  20. Complexity of aromatic ring-flip motions in proteins: Y97 ring dynamics in cytochrome c observed by cross-relaxation suppressed exchange NMR spectroscopy
  21. Hexagonal ice in pure water and biological NMR samples
  22. Erratum to: 13 C α CEST experiment on uniformly 13 C-labeled proteins
  23. Auto-inducing media for uniform isotope labeling of proteins with 15 N, 13 C and 2 H
Search Result: