Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: J Biomol NMR

Search In Journal Title:

Abbravation: Journal of Biomolecular NMR

Search In Journal Abbravation:

Publisher

Springer Netherlands

Search In Publisher:

DOI

10.1016/0092-8674(77)90059-9

Search In DOI:

ISSN

1573-5001

Search In ISSN:
Search In Title Of Papers:

Complete dissociation of the HIV1 gp41 ectodomain

Authors: Julien Roche John M Louis Annie Aniana Rodolfo Ghirlando Ad Bax
Publish Date: 2015/01/29
Volume: 61, Issue: 3-4, Pages: 235-248
PDF Link

Abstract

The envelope glycoprotein gp41 mediates the process of membrane fusion that enables entry of the HIV1 virus into the host cell Strong lipid affinity of the ectodomain suggests that its heptad repeat regions play an active role in destabilizing membranes by directly binding to the lipid bilayers and thereby lowering the freeenergy barrier for membrane fusion In such a model immediately following the shedding of gp120 the Nheptad and Cheptad helices dissociate and melt into the host cell and viral membranes respectively pulling the destabilized membranes into juxtaposition ready for fusion Postfusion reaching the final 6helix bundle 6HB conformation then involves competition between intermolecular interactions needed for formation of the symmetric 6HB trimer and the membrane affinity of gp41’s ectodomain including its membraneproximal regions Our solution NMR study of the structural and dynamic properties of three constructs containing the ectodomain of gp41 with and without its membraneproximal regions suggests that these segments do not form interhelical interactions until the very late steps of the fusion process Interactions between the polar termini of the heptad regions which are not associating with the lipid surface therefore may constitute the main driving force initiating formation of the final postfusion states The absence of significant intermolecular ectodomain interactions in the presence of dodecyl phosphocholine highlights the importance of trimerization of gp41’s transmembrane helix to prevent complete dissociation of the trimer during the course of fusionWe thank Drs James Baber and Jinfa Ying for technical support and acknowledge support from the Advanced Mass Spectrometry Core of the National Institute of Diabetes and Digestive and Kidney Diseases NIDDK This work was funded by the NIH Intramural Research Program of the NIDDK and by the Intramural AIDSTargeted Antiviral Program of the Office of the Director NIH


Keywords:

References


.
Search In Abstract Of Papers:
Other Papers In This Journal:

  1. Detection of nanosecond time scale side-chain jumps in a protein dissolved in water/glycerol solvent
  2. Statistical removal of background signals from high-throughput 1 H NMR line-broadening ligand-affinity screens
  3. Peak picking multidimensional NMR spectra with the contour geometry based algorithm CYPICK
  4. HNHC: a triple resonance experiment for correlating the H2, N1(N3) and C2 resonances in adenine nucleobases of 13 C-, 15 N-labeled RNA oligonucleotides
  5. NOESY-WaterControl: a new NOESY sequence for the observation of under-water protein resonances
  6. Insights into furanose solution conformations: beyond the two-state model
  7. Selective 1 H- 13 C NMR spectroscopy of methyl groups in residually protonated samples of large proteins
  8. Fast methionine-based solution structure determination of calcium-calmodulin complexes
  9. A procedure to validate and correct the 13 C chemical shift calibration of RNA datasets
  10. NMR solution structure of the acylphosphatase from Escherichia coli
  11. A rigid disulfide-linked nitroxide side chain simplifies the quantitative analysis of PRE data
  12. S3EPY: a Sparky extension for determination of small scalar couplings from spin-state-selective excitation NMR experiments
  13. Improved NMR experiments with 13 C-isotropic mixing for assignment of aromatic and aliphatic side chains in labeled proteins
  14. Out-and-back 13 C– 13 C scalar transfers in protein resonance assignment by proton-detected solid-state NMR under ultra-fast MAS
  15. Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization
  16. A probe to monitor performance of 15 N longitudinal relaxation experiments for proteins in solution
  17. Optimization of NMR spectroscopy of encapsulated proteins dissolved in low viscosity fluids
  18. Use of quantitative 1 H NMR chemical shift changes for ligand docking into barnase
  19. Resolution enhancement by homonuclear J-decoupling: application to three-dimensional solid-state magic angle spinning NMR spectroscopy
  20. Complexity of aromatic ring-flip motions in proteins: Y97 ring dynamics in cytochrome c observed by cross-relaxation suppressed exchange NMR spectroscopy
  21. Hexagonal ice in pure water and biological NMR samples
  22. Erratum to: 13 C α CEST experiment on uniformly 13 C-labeled proteins
  23. Auto-inducing media for uniform isotope labeling of proteins with 15 N, 13 C and 2 H

Search Result: