Authors: Nguyen Duc Quang Phan Thi Phuong Hoa Tran Thanh Da Phan Hoai Anh
Publish Date: 2008/08/12
Volume: 156, Issue: 1-4, Pages: 69-
Abstract
White Spot Syndrome Virus WSSV is the major and most serious pathogen in shrimp aquaculture industries By using a sensitive PCRbased detection technique followed by sequencing multiple PCR products for confirmation we address to the question of whether WSSV can persist in shrimp ponds and surrounding areas after an outbreak The seawater samples were taken from two shrimp ponds and surrounding canals in a coastal area in northern Vietnam Quang Ninh Province where the shrimps cultured in the two ponds had been killed due to a WSSV outbreak in April 2001 and the ponds were thereafter abandoned A total of 480 seawater samples 30 samples each for July and December of 2001 and 2002 were subjected to WSSV genome detection Although the detection rates of WSSV genome were generally gradually declined in seawater environments of both diseased shrimp ponds and surrounding canals WSSV was still detected with rates of more than 10 in the diseased ponds and lower in surrounding canals in December 2002 20 months after the WSSV outbreak
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