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Title of Journal: Mol Biotechnol

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Abbravation: Molecular Biotechnology

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Humana Press Inc

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DOI

10.1016/0002-9378(68)90324-4

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ISSN

1559-0305

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Heterologous Expression and Characterization of an

Authors: Erika Nahomy MarinoMarmolejo Antonio De LeónRodríguez Ana Paulina Barba de la Rosa Leticia Santos
Publish Date: 2008/12/05
Volume: 42, Issue: 1, Pages: 61-67
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Abstract

Analysis of the Thermoplasma acidophilum DSM 1728 genome identified two putative alcohol dehydrogenase ADH open reading frames showing 504 identity against each other The corresponding genes Ta0841 and Ta1316 encode proteins of 336 and 328 amino acids with molecular masses of 3648 and 3601 kDa respectively The genes were expressed in Escherichia coli and the recombinant enzymes were functionally assessed for activity Throughout the study only Ta1316 ADH resulted active in the oxidative reaction in the pH range 2–8 optimal pH 50 and temperatures from 25 to 90°C optimal 75°C This ADH catalyzes the oxidation of several alcohols such as ethanol methanol 2propanol butanol and pentanol during the reduction of the cofactor NAD+ The highest activity was found in the presence of ethanol producing optically pure acetaldehyde The specific enzyme activity of the purified Ta1316 ADH with ethanol as a substrate in the optimal conditions was 6287 U/mg

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  1. Production and Characterization of a Single-Chain Fv Antibody–Alkaline Phosphatase Fusion Protein Specific for Clenbuterol
  2. Improvement of Cephalosporin C Production by Recombinant DNA Integration in Acremonium chrysogenum
  3. Codon Optimization of the “Bos Taurus Chymosin” Gene for the Production of Recombinant Chymosin in Pichia pastoris
  4. Heterologous Overexpression, Purification and Characterisation of an Alcohol Dehydrogenase (ADH2) from Halobacterium sp. NRC-1
  5. Enhanced Extracellular Production of Heterologous Proteins in Bacillus subtilis by Deleting the C-terminal Region of the SecA Secretory Machinery
  6. Enhanced Production of Human Recombinant Proteins from CHO cells Grown to High Densities in Macroporous Microcarriers
  7. Molecular Cloning and Characterization of a Malic Enzyme Gene from the Oleaginous Yeast Lipomyces starkeyi
  8. Hydrophobic Substitution of Surface Residues Affects Lipase Stability in Organic Solvents
  9. Identification of Suitable Endogenous Normalizers for qRT-PCR Analysis of Plasma microRNA Expression in Essential Hypertension
  10. Biosynthesis of Resveratrol in Blastospore of the Macrofungus Tremella fuciformis
  11. Plasma Antioxidants and Human Aging: A Study on Healthy Elderly Tunisian Population
  12. Importance of the residue Asp 290 on chain length selectivity and catalytic efficiency of recombinant Staphylococcus simulans lipase expressed in E. coli
  13. Fahd Al-Mulla (ed). Formalin-Fixed Paraffin-Embedded Tissue. Methods and Protocols. Methods in Molecular Biology 724. The Humana Press, ISBN 978-1-61779-054-6
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  15. End Joining-Mediated Gene Expression in Mammalian Cells Using PCR-Amplified DNA Constructs that Contain Terminator in Front of Promoter
  16. Identification of Functional Regions in the Rhodospirillum rubrum l -Asparaginase by Site-Directed Mutagenesis
  17. Expression of an Acid Urease with Urethanase Activity in E. coli and Analysis of Urease Gene
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