Authors: Hiroshi Kakeshtia Yasushi Kageyama Katsutoshi Ara Katsuya Ozaki Kouji Nakamura
Publish Date: 2010/06/24
Volume: 46, Issue: 3, Pages: 250-257
Abstract
In this study we examined the effects of modifying the Cterminal region of the SecA protein on the production of heterologous proteins in Bacillus subtilis SecA was selected as a candidate among the components of the Sec system due to its ability to interact directly with both the precursors and membrane translocases A phylogenetic comparison demonstrated that the Cterminal region is not well conserved among eubacterial SecA proteins The deletion of the 61 amino acids at the Cterminal region led to an 83 increase in extracellular alkaliphilic Bacillus sp thermostable alkaline cellulase Egl237 activity Moreover the productivity of human interferon α hIFNα2b was increased by 22fold compared to the wildtype SecA by deletion of these 61 amino acids We indicated that the deletion of the Cterminal domain CTD of SecA enhanced the secretion of two different heterologous protein Egl237 and hIFNα2b This study provides a useful method to enhance the extracellular production of heterologous proteins in B subtilisThis study is the subproject ‘Development of a Technology for Creation of a Host Cell’ included within the industrial technology project ‘Development of a Generic Technology for Production Process Starting Productive Function’ of the Ministry of Economy Trade and Industry METI entrusted by the New Energy and Industrial Technology Development Organization NEDO Japan
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