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Title of Journal: Mol Biotechnol

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Abbravation: Molecular Biotechnology

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Humana Press Inc

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DOI

10.1002/chin.198909197

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1559-0305

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Enhanced Extracellular Production of Heterologous

Authors: Hiroshi Kakeshtia Yasushi Kageyama Katsutoshi Ara Katsuya Ozaki Kouji Nakamura
Publish Date: 2010/06/24
Volume: 46, Issue: 3, Pages: 250-257
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Abstract

In this study we examined the effects of modifying the Cterminal region of the SecA protein on the production of heterologous proteins in Bacillus subtilis SecA was selected as a candidate among the components of the Sec system due to its ability to interact directly with both the precursors and membrane translocases A phylogenetic comparison demonstrated that the Cterminal region is not well conserved among eubacterial SecA proteins The deletion of the 61 amino acids at the Cterminal region led to an 83 increase in extracellular alkaliphilic Bacillus sp thermostable alkaline cellulase Egl237 activity Moreover the productivity of human interferon α hIFNα2b was increased by 22fold compared to the wildtype SecA by deletion of these 61 amino acids We indicated that the deletion of the Cterminal domain CTD of SecA enhanced the secretion of two different heterologous protein Egl237 and hIFNα2b This study provides a useful method to enhance the extracellular production of heterologous proteins in B subtilisThis study is the subproject ‘Development of a Technology for Creation of a Host Cell’ included within the industrial technology project ‘Development of a Generic Technology for Production Process Starting Productive Function’ of the Ministry of Economy Trade and Industry METI entrusted by the New Energy and Industrial Technology Development Organization NEDO Japan


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Other Papers In This Journal:

  1. Production and Characterization of a Single-Chain Fv Antibody–Alkaline Phosphatase Fusion Protein Specific for Clenbuterol
  2. Improvement of Cephalosporin C Production by Recombinant DNA Integration in Acremonium chrysogenum
  3. Codon Optimization of the “Bos Taurus Chymosin” Gene for the Production of Recombinant Chymosin in Pichia pastoris
  4. Heterologous Overexpression, Purification and Characterisation of an Alcohol Dehydrogenase (ADH2) from Halobacterium sp. NRC-1
  5. Enhanced Production of Human Recombinant Proteins from CHO cells Grown to High Densities in Macroporous Microcarriers
  6. Molecular Cloning and Characterization of a Malic Enzyme Gene from the Oleaginous Yeast Lipomyces starkeyi
  7. Hydrophobic Substitution of Surface Residues Affects Lipase Stability in Organic Solvents
  8. Identification of Suitable Endogenous Normalizers for qRT-PCR Analysis of Plasma microRNA Expression in Essential Hypertension
  9. Biosynthesis of Resveratrol in Blastospore of the Macrofungus Tremella fuciformis
  10. Heterologous Expression and Characterization of an Alcohol Dehydrogenase from the Archeon Thermoplasma acidophilum
  11. Plasma Antioxidants and Human Aging: A Study on Healthy Elderly Tunisian Population
  12. Importance of the residue Asp 290 on chain length selectivity and catalytic efficiency of recombinant Staphylococcus simulans lipase expressed in E. coli
  13. Fahd Al-Mulla (ed). Formalin-Fixed Paraffin-Embedded Tissue. Methods and Protocols. Methods in Molecular Biology 724. The Humana Press, ISBN 978-1-61779-054-6
  14. The Peptide Derived from erbB2 Auto-Inhibitor Herstatin Shared in the Same Epitope and Function with Functional Antibody 2C4
  15. End Joining-Mediated Gene Expression in Mammalian Cells Using PCR-Amplified DNA Constructs that Contain Terminator in Front of Promoter
  16. Identification of Functional Regions in the Rhodospirillum rubrum l -Asparaginase by Site-Directed Mutagenesis
  17. Expression of an Acid Urease with Urethanase Activity in E. coli and Analysis of Urease Gene

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