Authors: Mohamed Hassan M Solayman Taimour Langaee Archanakumari Patel Lamia ElWakeel Manal ElHamamsy Osama Badary Julie A Johnson
Publish Date: 2016/01/21
Volume: 58, Issue: 3, Pages: 179-187
Abstract
Circulating microRNAs miRNAs are promising biomarkers for many diseases Quantitative reverse transcription polymerase chain reaction qRTPCR is a gold standard for miRNA expression profiling that requires proper data normalization Since there is no universal normalizer it is recommended to evaluate normalizers under every experimental condition This study describes the identification of suitable endogenous normalizers ENs for plasma miRNA expression in essential hypertension Expression levels of 5 candidate ENs and 2 plasma quality markers were determined by qRTPCR in plasma samples from 18 hypertensive patients and 10 healthy controls NormFinder GeNorm and DataAssist software programs were used to select the best ENs Expression levels of the 5 candidate ENs were also analyzed in urine samples from hypertensive patients and compared to the plasma samples of the hypertensive patients Among the analyzed candidates hsamiR92a3p was identified as the best EN and hsamiR215p and hsamiR165p as the next best Moreover hsamiR92a3p showed the most consistent expression between plasma and urine In conclusion this study showed that hsamiR92a3p hsamiR215p and hsamiR165p may be used as normalizers for plasma miRNA expression data in essential hypertension studies
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