Authors: LinZhi Kang Qiongjie Li JunFang Lin LiQiong Guo
Publish Date: 2015/03/13
Volume: 57, Issue: 7, Pages: 675-684
Abstract
Tremella fuciformis is a known edible macrofungus that has medicinal value It is widely cultivated in China and its products are distributed worldwide In this study a novel bioconversion system was established to produce resveratrol in the blastospore of T fuciformis bTf The expression vector ptro14clrs that contains 4coumaratecoenzyme A ligase gene 4cl and resveratrol synthase gene rs was transformed into bTf by LiAc/PEGmediated transformation PCR and southern blotting analysis verified the successful integration of the exogenous 4cl and rs genes into the genome of bTf HPLC analysis confirmed that two transformants can convert pcoumaric acid into resveratrol 092 and 083 μg/g resveratrol of dry weight within 7 days This study is the first to report about the transformation and expression of resveratrol biosynthetic genes in bTf This research is a significant step toward obtaining resveratrolproducing T fuciformis strains which not only satisfy the demand of resveratrol market but also expand the category of functional foodThis work was supported by the National Natural Science Foundation of China Grant Nos 31071837 31272217 31372116 the Projects of Science and Technology of Guangdong Province Grant Nos 2011B090400412 2012B020316004 2012A020100010 2013B010404041 and China National Engineering Research Center of Juncao Technology
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