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Title of Journal: Nano Res

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Abbravation: Nano Research

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Tsinghua Press

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DOI

10.1016/0008-8749(90)90110-d

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1998-0000

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Twophoton luminescence imaging of Emphasis Type=

Authors: Wei He Walter A Henne Qingshan Wei Yan Zhao Derek D Doorneweerd JiXin Cheng Philip S Low Alexander Wei
Publish Date: 2008/12/14
Volume: 1, Issue: 6, Pages: 450-456
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Abstract

Bacillus subtilis spores a simulant of Bacillus anthracis have been imaged by twophoton luminescence TPL microscopy using gold nanorods GNRs functionalized with a cysteineterminated homing peptide Control experiments using a peptide with a scrambled amino acid sequence confirmed that the GNR targeting was highly selective for the spore surfaces The high sensitivity of TPL combined with the high affinity of the peptide labels enables spores to be detected with high fidelity using GNRs at femtomolar concentrations It was also determined that GNRs are capable of significant TPL output even when irradiated at near infrared NIR wavelengths far from their longitudinal plasmon resonance LPR permitting considerable flexibility in the choice of GNR aspect ratio or excitation wavelength for TPL imagingThis article is published under an open access license Please check the Copyright Information section for details of this license and what reuse is permitted If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and reuse information please contact the Rights and Permissions team

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