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Title of Journal: Funct Integr Genomics

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Abbravation: Functional & Integrative Genomics

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Springer-Verlag

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DOI

10.1016/0009-8981(71)90379-2

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1438-7948

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Phytoremediation and phytosensing of chemical cont

Authors: Murali R Rao Matthew D Halfhill Laura G Abercrombie Priya Ranjan Jason M Abercrombie Julia S Gouffon Arnold M Saxton C Neal Stewart
Publish Date: 2009/06/19
Volume: 9, Issue: 4, Pages: 537-
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Abstract

High explosives such as hexahydro135trinitro135triazine RDX and 246trinitrotoluene TNT are important contaminants in the environment and phytoremediation has been viewed as a costeffective abatement There remains however an insufficient knowledgebase about how plants respond to explosives especially in the steady state Microarray analysis was conducted on Arabidopsis thaliana that were grown in Murashige and Skoog media containing steadystate levels of 05 mM RDX or 20 μM TNT to study the effect of these compounds on its transcriptional profile Our results for both RDX and TNT were consistent with the existing theory for xenobiotic metabolism in plants Among the genes that were differentially expressed included oxidoreductases cytochrome P450s transferases transporters and several unknown expressed proteins We discuss the potential role of upregulated genes in plant metabolism phytoremediation and phytosensing Phytosensing the detection of field contamination using plants is an end goal of this projectWe would like to thank Pradeep Chimakurthy for the help on Affymetrix data analysis and all the members of Stewart lab for their support Also we would like to thank the reviewers for their constructive comments and suggestions We are grateful for the funding by DIAAFMIC and the Tennessee Agricultural Experiment Station


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