Emphasis Type="Italic"Os11Gsk/Emphasis gene fr

Authors: Sudhakar Thalapati Anil K Batchu Sarla Neelamraju Rajeshwari Ramanan

Publish Date: 2012/02/25

Volume: 12, Issue: 2, Pages: 277-289

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Abstract

Chromosomal segments from wild rice species Oryza rufipogon introgressed into an elite indica rice restorer line KMR3 using molecular markers resulted in significant increase in yield Here we report the transcriptome analysis of flag leaves and fully emerged young panicles of one of the high yielding introgression lines IL507 in comparison to KMR3 A 66fold upregulated gene Os11Gsk which showed no transcript in KMR3 was highly expressed in O rufipogon and IL507 A 5kb genomic region including Os11Gsk and its flanking regions could be PCR amplified only from IL507 O rufipogon japonica varieties of riceNipponbare and Kitaake but not from the indica varieties KMR3 and Taichung Native1 Three sister lines of IL507 yielding higher than KMR3 showed presence of Os11Gsk whereas the gene was absent in three other ILs from the same cross having lower yield than KMR3 indicating an association of the presence of Os11Gsk with high yield Southern analysis showed additional bands in the genomic DNA of O rufipogon and IL507 with Os11Gsk probe Genomic sequence analysis of ten highly coexpressed differentially regulated genes revealed that two upregulated genes in IL507 were derived from O rufipogon and most of the downregulated genes were either from KMR3 or common to KMR3 IL507 and O rufipogon Thus we show that Os11Gsk is a wild ricederived gene introduced in KMR3 background and increases yield either by regulating expression of functional genes sharing homology with it or by causing epigenetic modifications in the introgression lineThis work and ST were supported by a DBT grant to NS DBT NoBT/AD/FG2PHII2009 AKB was funded by Indian Council for Agricultural Research project 3019 NPTC/FG/05/2672/33 We acknowledge the contributions of A Prasad Babu C Surendhar Reddy and BP Mallikarjuna Swamy in earlier work on developing and evaluating introgression lines and marker aided selection We thank Project Director DRR for the encouragement We thank G Haritha and N Naga Deepthi for help in identifying polymorphic markers The authors are thankful to the Sequencing laboratory CCMB India for providing the sequencing facility used for this study

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