Journal Title
Title of Journal: Funct Integr Genomics
|
Abbravation: Functional & Integrative Genomics
|
Publisher
Springer-Verlag
|
|
|
|
Authors: Lei Zhao Liping Gao Hongxue Wang Xiaotian Chen Yunsheng Wang Hua Yang Chaoling Wei Xiaochun Wan Tao Xia
Publish Date: 2012/11/27
Volume: 13, Issue: 1, Pages: 75-98
Abstract
R2R3MYB bHLH and WD40 proteins have been shown to control multiple enzymatic steps in the biosynthetic pathway responsible for the production of flavonoids important secondary metabolites in Camellia sinensis Few related transcription factor genes have been documented The presence of R2R3MYB bHLH and WD40 were statistically and bioinformatically analyzed on 127094 C sinensis transcriptome unigenes resulting in identification of 73 49 and 134 genes respectively C sinensis phylogenetic trees were constructed for R2R3MYB and bHLH proteins using previous Arabidopsis data and further divided into 27 subgroups Sg and 32 subfamilies Motifs in some R2R3MYB subgroups were redefined Furthermore Sg26 and Sg27 were expanded compared to Arabidopsis data and bHLH proteins in C sinensis were grouped into nine subfamilies According to the functional annotation of Arabidopsis flavonoid biosynthesis in C sinensis was predicted to include R2R3MYB genes in Sg4 6 Sg5 2 and Sg7 1 as well as bHLH genes in subfamily 2 2 and subfamily 24 5 The wide evolutionary gap prevented phylogenetic analysis of WD40s however a single gene CsWD401 was observed to share 804 sequence homogeny with AtTTG1 Analysis of CsMYB41 CsMYB42 CsMYB43 CsMYB44 CsMYB51 and CsMYB52 revealed the interaction motif DELx2RKx3Lx6Lx3R potentially contributing to the specificity of the bHLH partner in the stable MYB–bHLH complex Fulllength endtoend polymerase chain reaction PCR and quantitative reverse transcriptase qRTPCR were used to validate selected genes and generate relative expression ratio profiles in C sinensis leaves by developmental stage and treatment conditions including hormone and wound treatments Potential target binding sites were predictedThis work was supported by the Natural Science Foundation of China 30972401 31170647 and 31170282 Natural Science Foundation of Anhui Province 11040606M73 Collegiate Natural Science Foundation of Anhui Province KJ2012A110 Program for Changjiang Scholars and Innovative Research Team in University IRT1101 and Major Project of Chinese National Programmes for Fundamental Research and Development 2012CB722903
Keywords:
.
|
Other Papers In This Journal:
- Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium
- miRNA-based drought regulation in wheat
- Comparative genomics and functional analysis of the NiaP family uncover nicotinate transporters from bacteria, plants, and mammals
- Integrated protein network and microarray analysis to identify potential biomarkers after myocardial infarction
- Comparative analysis of expressed sequence tags from different organs of Vitis vinifera L.
- Genome-wide analysis of intronless genes in rice and Arabidopsis
- Single-copy genes define a conserved order between rice and wheat for understanding differences caused by duplication, deletion, and transposition of genes
- Identification of candidate genes JcARF19 and JcIAA9 associated with seed size traits in Jatropha
- Genome-wide expression profiling in Geobacter sulfurreducens : identification of Fur and RpoS transcription regulatory sites in a rel Gsu mutant
- Empirical Bayes estimation of gene-specific effects in micro-array research
- Phytoremediation and phytosensing of chemical contaminants, RDX and TNT: identification of the required target genes
- Os11Gsk gene from a wild rice, Oryza rufipogon improves yield in rice
- Genomic sequencing reveals gene content, genomic organization, and recombination relationships in barley
|