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Title of Journal: Funct Integr Genomics

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Abbravation: Functional & Integrative Genomics

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Springer-Verlag

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DOI

10.1002/jcp.20474

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1438-7948

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The R2R3MYB bHLH WD40 and related transcriptio

Authors: Lei Zhao Liping Gao Hongxue Wang Xiaotian Chen Yunsheng Wang Hua Yang Chaoling Wei Xiaochun Wan Tao Xia
Publish Date: 2012/11/27
Volume: 13, Issue: 1, Pages: 75-98
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Abstract

R2R3MYB bHLH and WD40 proteins have been shown to control multiple enzymatic steps in the biosynthetic pathway responsible for the production of flavonoids important secondary metabolites in Camellia sinensis Few related transcription factor genes have been documented The presence of R2R3MYB bHLH and WD40 were statistically and bioinformatically analyzed on 127094 C sinensis transcriptome unigenes resulting in identification of 73 49 and 134 genes respectively C sinensis phylogenetic trees were constructed for R2R3MYB and bHLH proteins using previous Arabidopsis data and further divided into 27 subgroups Sg and 32 subfamilies Motifs in some R2R3MYB subgroups were redefined Furthermore Sg26 and Sg27 were expanded compared to Arabidopsis data and bHLH proteins in C sinensis were grouped into nine subfamilies According to the functional annotation of Arabidopsis flavonoid biosynthesis in C sinensis was predicted to include R2R3MYB genes in Sg4 6 Sg5 2 and Sg7 1 as well as bHLH genes in subfamily 2 2 and subfamily 24 5 The wide evolutionary gap prevented phylogenetic analysis of WD40s however a single gene CsWD401 was observed to share 804  sequence homogeny with AtTTG1 Analysis of CsMYB41 CsMYB42 CsMYB43 CsMYB44 CsMYB51 and CsMYB52 revealed the interaction motif DELx2RKx3Lx6Lx3R potentially contributing to the specificity of the bHLH partner in the stable MYB–bHLH complex Fulllength endtoend polymerase chain reaction PCR and quantitative reverse transcriptase qRTPCR were used to validate selected genes and generate relative expression ratio profiles in C sinensis leaves by developmental stage and treatment conditions including hormone and wound treatments Potential target binding sites were predictedThis work was supported by the Natural Science Foundation of China 30972401 31170647 and 31170282 Natural Science Foundation of Anhui Province 11040606M73 Collegiate Natural Science Foundation of Anhui Province KJ2012A110 Program for Changjiang Scholars and Innovative Research Team in University IRT1101 and Major Project of Chinese National Programmes for Fundamental Research and Development 2012CB722903


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