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Title of Journal: Food Bioprocess Technol

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Abbravation: Food and Bioprocess Technology

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Springer-Verlag

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DOI

10.1016/0002-9416(57)90146-x

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1935-5149

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Extraction and Purification of Lipoxygenase from S

Authors: M C Lakshmi M C Madhusudhan K S M S Raghavarao
Publish Date: 2009/10/24
Volume: 5, Issue: 1, Pages: 193-199
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Abstract

The purification of lipoxygenase in soybeans richest known source was attempted by precipitation using polyethylene glycol followed by aqueous twophase extraction ATPE The preliminary purification by precipitation was standardized which resulted in twofold purification The influence of process parameters such as phase forming salt molecular weight of phase forming polymer tie line length and phase volume ratio on the partitioning behavior of lipoxygenase during ATPE was investigated The aqueous twophase system of polyethylene glycol 6000 and ammonium sulfate with inherent pH of 52 was found to be most suitable The tie line length of 4125 and volume ratio of 17 have resulted in 125 activity recovery with the overall purification factor of 438 compared to crude enzyme extract

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