Authors: Maryam Hashemi Seyed Abbas Shojaosadati Seyed Hadi Razavi Seyyed Mohammad Mousavi Khosro Khajeh Mohammad Safari
Publish Date: 2010/09/14
Volume: 5, Issue: 3, Pages: 1093-1099
Abstract
The performance of temperatureshift strategies on the production of αamylase by Bacillus sp in solidstate fermentation systems was investigated Switching the temperature from an initial set point 37 °C 40 °C or 45 °C to 28 °C increased the production of αamylase significantly in all treatments with different rates The effects of having a constant incubation temperature and performing the temperatureshift operation from higher to lower temperatures 37 °C 40 °C or 45 °C to 32 °C and vice versa were also studied The results showed that the αamylase biosynthesis could successfully be improved by shifting the incubation temperature from 40 °C for the initial 20 h of fermentation to 32 °C after a span of 20–48 h An enhancement of 295 in the production of αamylase after 48 h was recorded compared to the treatment that was shifted from 37 °C to 32 °C This finding is particularly attractive for largescale industrial fermentation from an economic point of viewThis work was financially supported by the Department of Food Science and Engineering Agricultural Engineering and Technology Faculty the University of Tehran and the Agricultural Biotechnology Research Institute of Iran ABRII The authors would thank Dr Khajeh Department of Biochemistry and Biophysics Faculty of Science Tarbiat Modares University for providing the bacterial strain
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