Authors: Wei Zhao Ruijin Yang
Publish Date: 2010/11/12
Volume: 5, Issue: 5, Pages: 1706-1714
Abstract
Ovalbumin OVA bovine serum albumin BSA and a mixture of the two proteins OVA + BSA in solution were exposed to pulsed electric field PEF to investigate the protein interaction and aggregation The results demonstrated the selfaggregation of OVA through disulfide bond due to the exposure of sulfhydryl groups and intermolecular disulfide interactions when PEF intensity exceeded 25 kV cm−1 However no protein selfaggregation of BSA was observed under PEF treatments of 20 to 35 kV cm−1 which might be ascribed to the less sulfhydryl groups in BSA molecule The mixture of the two proteins OVA + BSA were also subjected to the same PEF treatments The results showed both OVA and BSA molecules were involved in the protein interaction and aggregation when PEF intensity exceeded 25 kV cm−1 The BSA which was not sensitive to PEF was incorporated covalently into protein aggregates through disulfide crosslinks with the other proteins under PEFThe authors gratefully acknowledge the supported by Project 31000829 20772049 of the National Natural Science Foundation of PR China National and Fundamental Research Funds for the Central Universities JUSRP20910 This study was also supported by Project BK2010148 of Jiangsu Provincial National Natural Science Foundation Jiangsu Provincial Agriculture Selfrenovation Foundation CX10231 and 111 projectB07029 Program for Changjiang Scholars and Innovative Research Team in University
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